Detailed information NTG0411603

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GSF12_RS03020
• Genome accession: NZ_CP047226
• Coordinates: 651266..652318 (+)
• Length: 350 a.a.
• NCBI ID: WP_159374323.1
• Uniprot ID: A0A6P1KA87
• Organism: Moraxella osloensis strain YV1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 646266..657318

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GSF12_RS03000 (GSF12_03000)	-	646722..647240 (+)	519	WP_096488583.1	DUF6586 family protein	-
GSF12_RS03005 (GSF12_03005)	-	647356..650082 (-)	2727	WP_159374321.1	penicillin-binding protein 1A	-
GSF12_RS03010 (GSF12_03010)	-	650240..650740 (-)	501	WP_159374277.1	IS630 family transposase	-
GSF12_RS03015 (GSF12_03015)	-	650773..651153 (-)	381	WP_159374322.1	helix-turn-helix domain-containing protein	-
GSF12_RS03020 (GSF12_03020)	pilM	651266..652318 (+)	1053	WP_159374323.1	pilus assembly protein PilM	Machinery gene
GSF12_RS03025 (GSF12_03025)	-	652318..652989 (+)	672	WP_159374324.1	PilN domain-containing protein	-
GSF12_RS03030 (GSF12_03030)	-	652992..653753 (+)	762	WP_201450424.1	type 4a pilus biogenesis protein PilO	-
GSF12_RS03035 (GSF12_03035)	-	653740..654273 (+)	534	WP_159374325.1	pilus assembly protein PilP	-
GSF12_RS03040 (GSF12_03040)	pilQ	654291..656624 (+)	2334	WP_159374326.1	type IV pilus secretin family protein	-
GSF12_RS03045 (GSF12_03045)	-	656733..657269 (+)	537	WP_159374327.1	shikimate kinase	-

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 38350.65 Da
• Isoelectric Point: 4.5359

>NTDB_id=411603 GSF12_RS03020 WP_159374323.1 651266..652318(+) (pilM) [Moraxella osloensis strain YV1]
MRLFTKNNKQVVGVDITTTAIKMVEITRQQGLFHLRNYGIEPLPRGAVVDKNIVDIDAVSDALARLSRGSSFSSKHIATA
VSGSSVISKIIEMETDLTELERESRIRLDADQYIPYPLSEVNLDFEVIGPSATPDMCKVLLVASRTENVDQRVDVGALAG
LETKIVDVEAYAVERAFQLIVDSLPGQPEHVALIDIGHSQTTLYIAKDGEFVYSREQLFGGAQLTESIQSRYGLTFEEAM
INKRTLSLPDDYYTDVLMPFLDAIVQQITRSLQFYFSSSQNSQVDYILLGGGSASIAGLASMVEQKLGTRAGIANAFSNM
TINNHINTELLIMDAPSLIAACGLALRSFD

Nucleotide

• Length: 1053 bp

>NTDB_id=411603 GSF12_RS03020 WP_159374323.1 651266..652318(+) (pilM) [Moraxella osloensis strain YV1]
GTGAGGTTATTTACAAAAAACAACAAGCAAGTCGTTGGGGTAGATATCACCACCACTGCGATAAAAATGGTTGAAATAAC
ACGGCAGCAAGGTCTATTTCATTTAAGAAATTACGGCATTGAACCGTTACCCCGCGGTGCAGTTGTGGATAAAAATATTG
TAGATATTGACGCGGTCAGTGACGCCCTCGCAAGATTATCCCGCGGTAGCAGTTTTTCTAGTAAACATATCGCGACAGCC
GTATCAGGGTCTTCGGTTATTAGCAAAATTATTGAGATGGAAACGGATCTCACAGAGCTTGAAAGAGAATCTCGTATCCG
TCTAGATGCTGACCAATATATTCCCTATCCTTTATCAGAAGTTAATTTAGATTTTGAAGTGATAGGTCCTTCAGCCACGC
CTGATATGTGTAAGGTATTGTTAGTTGCCTCTCGCACTGAAAACGTCGACCAGCGTGTCGATGTGGGTGCTTTGGCGGGA
CTGGAAACTAAAATTGTGGATGTAGAGGCTTACGCAGTTGAACGCGCCTTTCAATTGATAGTCGATAGCTTGCCAGGTCA
ACCTGAGCATGTAGCCCTCATTGATATCGGGCACAGCCAAACCACACTTTATATTGCAAAAGACGGGGAGTTTGTATACA
GCCGAGAGCAGCTTTTTGGTGGCGCCCAGTTAACTGAAAGTATTCAAAGTCGTTATGGTTTAACGTTCGAGGAGGCGATG
ATTAATAAGCGCACCTTGTCATTGCCAGATGATTACTATACGGATGTATTAATGCCATTTCTAGATGCCATTGTGCAGCA
AATCACCCGCTCGCTGCAGTTTTATTTTTCATCAAGCCAAAATAGCCAAGTAGACTATATTTTGTTGGGTGGGGGTAGTG
CCAGCATTGCGGGTCTAGCAAGCATGGTTGAACAAAAACTTGGTACGCGCGCAGGTATTGCCAATGCATTTTCAAATATG
ACGATTAATAACCATATCAACACTGAATTACTGATTATGGATGCGCCAAGCTTAATAGCAGCTTGTGGCTTGGCGTTACG
GAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-349)

3D structure

• AlphaFold DB: A0A6P1KA87

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.852	100	0.52
comM	Acinetobacter nosocomialis M2	50.997	100	0.511
comM	Acinetobacter baylyi ADP1	48.433	100	0.486
pilM	Legionella pneumophila strain ERS1305867	42.776	100	0.431