Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   H5C78_RS19315 Genome accession   NZ_CP059917
Coordinates   4020729..4022222 (-) Length   497 a.a.
NCBI ID   WP_000249319.1    Uniprot ID   -
Organism   Escherichia coli strain 64.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4015729..4027222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5C78_RS19285 (H5C78_19285) gspK 4016523..4017500 (-) 978 WP_000633196.1 type II secretion system minor pseudopilin GspK -
  H5C78_RS19290 (H5C78_19290) gspJ 4017497..4018102 (-) 606 WP_001318033.1 type II secretion system minor pseudopilin GspJ -
  H5C78_RS19295 (H5C78_19295) gspI 4018099..4018470 (-) 372 WP_000820091.1 type II secretion system minor pseudopilin GspI -
  H5C78_RS19300 (H5C78_19300) gspH 4018467..4019030 (-) 564 WP_001115149.1 type II secretion system minor pseudopilin GspH -
  H5C78_RS19305 (H5C78_19305) gspG 4019034..4019489 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  H5C78_RS19310 (H5C78_19310) gspF 4019506..4020729 (-) 1224 WP_025491771.1 type II secretion system inner membrane protein GspF -
  H5C78_RS19315 (H5C78_19315) pilB/pilB1 4020729..4022222 (-) 1494 WP_000249319.1 type II secretion system ATPase GspE Machinery gene
  H5C78_RS19320 (H5C78_19320) gspD 4022222..4024282 (-) 2061 WP_001318034.1 type II secretion system secretin GspD -
  H5C78_RS19325 (H5C78_19325) gspC 4024312..4025271 (-) 960 WP_001696202.1 type II secretion system protein GspC -
  H5C78_RS19330 (H5C78_19330) gspS2 4025289..4025699 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  H5C78_RS19335 (H5C78_19335) pilD 4025765..4026574 (-) 810 WP_001318036.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55178.36 Da        Isoelectric Point: 5.9081

>NTDB_id=411563 H5C78_RS19315 WP_000249319.1 4020729..4022222(-) (pilB/pilB1) [Escherichia coli strain 64.1]
MVPVAQETTANTVRLPYSFSRRFGLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSVPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=411563 H5C78_RS19315 WP_000249319.1 4020729..4022222(-) (pilB/pilB1) [Escherichia coli strain 64.1]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTGGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTACAGGAGCTACAGCGGGGGCTGA
ATGCGCCCTTTACACTGCGGCAAATCGACGAGGCTGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTTGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGTACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGCACCGCTATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45.25

80.483

0.364

Multiple sequence alignment