Detailed information NTG0411182

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H0H24_RS17765
• Genome accession: NZ_CP059729
• Coordinates: 3737567..3738685 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain AbCTX13
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3732567..3743685

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0H24_RS17735 (H0H24_17710)	-	3733396..3734244 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
H0H24_RS17740 (H0H24_17715)	-	3734497..3734952 (+)	456	WP_000782976.1	bacteriohemerythrin	-
H0H24_RS17745 (H0H24_17720)	-	3735105..3736172 (+)	1068	WP_029776981.1	membrane protein	-
H0H24_RS17750 (H0H24_17725)	-	3736172..3736495 (+)	324	WP_000442585.1	RnfH family protein	-
H0H24_RS17755 (H0H24_17730)	-	3736550..3736948 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H0H24_RS17760 (H0H24_17735)	fur	3737060..3737497 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H0H24_RS17765 (H0H24_17740)	pilU	3737567..3738685 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H0H24_RS17770 (H0H24_17745)	pilT	3738713..3739750 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H0H24_RS17775 (H0H24_17750)	-	3739877..3740569 (+)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=411182 H0H24_RS17765 WP_000347036.1 3737567..3738685(-) (pilU) [Acinetobacter baumannii strain AbCTX13]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=411182 H0H24_RS17765 WP_000347036.1 3737567..3738685(-) (pilU) [Acinetobacter baumannii strain AbCTX13]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392