Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GQ674_RS15295 Genome accession   NZ_CP047135
Coordinates   3388303..3389361 (-) Length   352 a.a.
NCBI ID   WP_038686523.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3383303..3394361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ674_RS15275 (GQ674_15275) - 3384269..3386248 (-) 1980 WP_159497756.1 type IV pilus secretin PilQ family protein -
  GQ674_RS15280 (GQ674_15280) - 3386269..3386802 (-) 534 WP_128097583.1 pilus assembly protein PilP -
  GQ674_RS15285 (GQ674_15285) pilO 3386799..3387473 (-) 675 WP_128097596.1 type 4a pilus biogenesis protein PilO -
  GQ674_RS15290 (GQ674_15290) - 3387470..3388303 (-) 834 WP_159497757.1 PilN domain-containing protein -
  GQ674_RS15295 (GQ674_15295) pilM 3388303..3389361 (-) 1059 WP_038686523.1 pilus assembly protein PilM Machinery gene
  GQ674_RS15300 (GQ674_15300) - 3389586..3392006 (+) 2421 WP_159497758.1 penicillin-binding protein 1A -
  GQ674_RS15305 (GQ674_15305) - 3392295..3392939 (+) 645 WP_159497759.1 hypothetical protein -
  GQ674_RS15310 (GQ674_15310) - 3392975..3393826 (-) 852 WP_159497760.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37704.24 Da        Isoelectric Point: 4.4460

>NTDB_id=410928 GQ674_RS15295 WP_038686523.1 3388303..3389361(-) (pilM) [Stenotrophomonas sp. 364]
MGLIPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVNRSGTKARHAAAA
VAGSAVITKLIPMPADLDENDMEAQVELEAVNYIPYPIEEVNLDFEVLGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLTARVMDVEAFAVENAFALVASELPIASDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPEMVEEQLGVATVVANPLA
QMTLGPKVQAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=410928 GQ674_RS15295 WP_038686523.1 3388303..3389361(-) (pilM) [Stenotrophomonas sp. 364]
GTGGGGCTTATCCCAAAAAGCCAGTCGCCGCTTATTGGCGTCGACATCAGCTCGACTGCAGTAAAGCTCTTGCAGCTTTC
CCGCAGTGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCACTTCCGCCGAATGCGGTCGTGGAAAAGAACATCG
TGGAAGTGGAGGCCGTGGGTGAGGCTATCCGCCGCGCCGTGAACCGTTCCGGTACGAAGGCCCGGCACGCTGCCGCAGCC
GTCGCCGGCTCGGCGGTGATCACCAAGCTGATCCCGATGCCGGCCGATCTGGACGAGAACGACATGGAAGCCCAGGTCGA
GCTCGAGGCGGTCAACTACATCCCGTACCCGATCGAGGAAGTGAACCTCGACTTCGAGGTGCTGGGTGCCATTCCCAACA
ACCCGGAAATGGTCCAGGTGCTGTTGGCGGCGTCGCGGTCGGAGAACGTGGAGCTGCGCCAGTCGGCGCTGGAGCTCGGC
GGGCTGACCGCCCGGGTCATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTTTGCCCTGGTCGCCAGCGAGCTGCCGAT
CGCCAGCGATGGCGTGGTCGCCCTGGTGGACATCGGTGCCACCATGACCACCCTGAACGTCCTGCGCGGCGGGCGGAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACCGACGAAGTGATGCGCCGCTACGGCCTCACCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAGGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCCGGTAGCGAATTCAACCGTGTGGACCACATCGTGCTGGCCGGCG
GGTGCGCTGCGCTGGCCGGCCTGCCGGAGATGGTGGAAGAACAGCTGGGCGTGGCCACCGTGGTGGCCAACCCGCTGGCA
CAGATGACCCTGGGTCCCAAGGTCCAGGCGCATGCCCTGGCCCAGGACGCCCCGGCGCTGATGATTGCCACCGGTCTGGC
GCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.852

100

0.509
  comM Acinetobacter nosocomialis M2

50.568

100

0.506
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

47.009

99.716

0.469

Multiple sequence alignment