NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	H1Q68_RS14455	Genome accession	NZ_CP059448
Coordinates	3187849..3188508 (+)	Length	219 a.a.
NCBI ID	WP_053256075.1	Uniprot ID	A0A1S2V4Q4
Organism	Pseudomonas fluorescens strain 2020WEIHUA_J
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3182849..3193508

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1Q68_RS14430 (H1Q68_14405)	-	3182941..3183642 (-)	702	WP_053256080.1	VIT family protein	-
H1Q68_RS14435 (H1Q68_14410)	-	3183686..3184642 (-)	957	WP_053256079.1	FAD:protein FMN transferase	-
H1Q68_RS14440 (H1Q68_14415)	-	3184632..3186812 (-)	2181	WP_053256078.1	PepSY domain-containing protein	-
H1Q68_RS14445 (H1Q68_14420)	-	3186838..3187308 (-)	471	WP_053256077.1	DUF2271 domain-containing protein	-
H1Q68_RS14450 (H1Q68_14425)	-	3187394..3187717 (-)	324	WP_053256076.1	PepSY domain-containing protein	-
H1Q68_RS14455 (H1Q68_14430)	ciaR	3187849..3188508 (+)	660	WP_053256075.1	response regulator transcription factor	Regulator
H1Q68_RS14460 (H1Q68_14435)	-	3188505..3189851 (+)	1347	WP_053256074.1	sensor histidine kinase	-
H1Q68_RS14465 (H1Q68_14440)	-	3189930..3190346 (-)	417	WP_053256073.1	DUF6691 family protein	-
H1Q68_RS14470 (H1Q68_14445)	-	3190349..3190783 (-)	435	WP_053256072.1	YeeE/YedE family protein	-
H1Q68_RS14475 (H1Q68_14450)	-	3190960..3191100 (-)	141	WP_073637509.1	DUF2256 domain-containing protein	-
H1Q68_RS14480 (H1Q68_14455)	-	3191097..3192635 (-)	1539	WP_053256071.1	cryptochrome/photolyase family protein	-
H1Q68_RS14485 (H1Q68_14460)	-	3192650..3193360 (-)	711	WP_053256070.1	SDR family NAD(P)-dependent oxidoreductase	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24415.85 Da Isoelectric Point: 5.1358

                                >NTDB_id=410366 H1Q68_RS14455 WP_053256075.1 3187849..3188508(+) (ciaR) [Pseudomonas fluorescens strain 2020WEIHUA_J]

MRVLLVEDAPGLGEAVREQIADDGHAVDWVQRLNHASNSVRTTPYDLILLDLMLPDGRGLDFLREQRSAGNVTPVIILTA

QDQISDRIAGLNAGADDYLVKPFDLFELSARVAAVARRYSGNPNPQIKLGELQIDMNARTVQRAGITVDLTAREWALFEA

FVQRPNALLSKSQLEERLYAFGAEIESNTIEVYISRLRKKLGRDLVETVRGMGYRLTPV

Nucleotide

Download Length: 660 bp

                                >NTDB_id=410366 H1Q68_RS14455 WP_053256075.1 3187849..3188508(+) (ciaR) [Pseudomonas fluorescens strain 2020WEIHUA_J]

ATGCGGGTTTTATTGGTTGAGGATGCCCCCGGGCTGGGGGAGGCAGTGCGCGAGCAGATCGCCGACGACGGCCATGCGGT

TGATTGGGTGCAGCGCCTGAACCACGCAAGCAATAGCGTGCGCACCACGCCTTATGACTTGATCCTGCTGGACCTGATGT

TGCCGGACGGTCGCGGGTTGGACTTTTTGCGCGAGCAGCGCTCAGCAGGCAACGTGACCCCGGTGATTATCCTCACCGCC

CAGGACCAAATATCCGATCGCATTGCCGGCCTCAATGCCGGCGCTGACGACTACTTGGTCAAACCGTTCGACCTGTTTGA

ACTCTCGGCCCGGGTGGCAGCCGTGGCCCGACGTTACAGCGGTAACCCCAACCCGCAGATCAAACTCGGTGAACTGCAGA

TCGACATGAACGCTCGCACAGTCCAGCGCGCCGGTATCACCGTGGACTTGACCGCACGTGAGTGGGCGCTGTTCGAGGCA

TTCGTGCAGCGCCCCAATGCGTTGCTGTCGAAGTCCCAGCTCGAAGAGCGCCTGTACGCTTTTGGCGCGGAGATCGAGAG

CAACACCATTGAGGTCTATATCAGTCGCCTGCGTAAAAAACTGGGACGCGACCTGGTTGAGACCGTTCGTGGCATGGGTT

ATCGGTTGACGCCAGTATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(147-216)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A1S2V4Q4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	38.767	100	0.402
ciaR	Streptococcus pneumoniae D39	38.767	100	0.402
ciaR	Streptococcus pneumoniae R6	38.767	100	0.402
ciaR	Streptococcus pneumoniae TIGR4	38.767	100	0.402
ciaR	Streptococcus mutans UA159	37.946	100	0.388
covR	Lactococcus lactis subsp. lactis strain DGCC12653	35.747	100	0.361

Detailed information NTG0410366