Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GRB21_RS27400 Genome accession   NZ_CP047073
Coordinates   5839416..5840480 (-) Length   354 a.a.
NCBI ID   WP_011105329.1    Uniprot ID   Q87V09
Organism   Pseudomonas syringae pv. tomato strain delta X     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5834416..5845480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRB21_RS27375 aroK 5834970..5835488 (-) 519 WP_007245596.1 shikimate kinase AroK -
  GRB21_RS27380 pilQ 5835493..5837598 (-) 2106 WP_005765499.1 type IV pilus secretin PilQ Machinery gene
  GRB21_RS27385 pilP 5837646..5838173 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  GRB21_RS27390 pilO 5838170..5838793 (-) 624 WP_005765501.1 type 4a pilus biogenesis protein PilO -
  GRB21_RS27395 - 5838790..5839416 (-) 627 WP_005765503.1 PilN domain-containing protein -
  GRB21_RS27400 pilM 5839416..5840480 (-) 1065 WP_011105329.1 pilus assembly protein PilM Machinery gene
  GRB21_RS27405 - 5840690..5843131 (+) 2442 WP_412177578.1 penicillin-binding protein 1A -
  GRB21_RS27415 - 5843491..5844759 (-) 1269 WP_003378855.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38131.68 Da        Isoelectric Point: 4.7049

>NTDB_id=410172 GRB21_RS27400 WP_011105329.1 5839416..5840480(-) (pilM) [Pseudomonas syringae pv. tomato strain delta X]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLIKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=410172 GRB21_RS27400 WP_011105329.1 5839416..5840480(-) (pilM) [Pseudomonas syringae pv. tomato strain delta X]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTACTGATCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCGGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
CAAGCTTGAAGCTGATCAGTACATCCCTTACCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCATGGTC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGTTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAGGTGCTGGATCCCTTCAAGGAAGC
GCTGGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCGTCGATCTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACACCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGTTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCACCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87V09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466

Multiple sequence alignment