Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GNX18_RS12500 Genome accession   NZ_CP046948
Coordinates   3064939..3066015 (-) Length   358 a.a.
NCBI ID   WP_166218514.1    Uniprot ID   A0A6G8DFR4
Organism   Microbulbifer sp. SH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3059939..3071015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX18_RS12475 (GNX18_12460) aroK 3060461..3060985 (-) 525 WP_166218510.1 shikimate kinase AroK -
  GNX18_RS12480 (GNX18_12465) pilQ 3060982..3063207 (-) 2226 WP_166218512.1 type IV pilus secretin PilQ -
  GNX18_RS12485 (GNX18_12470) - 3063210..3063737 (-) 528 WP_138235203.1 pilus assembly protein PilP -
  GNX18_RS12490 (GNX18_12475) - 3063734..3064354 (-) 621 WP_138235204.1 type 4a pilus biogenesis protein PilO -
  GNX18_RS12495 (GNX18_12480) - 3064357..3064935 (-) 579 WP_138235205.1 PilN domain-containing protein -
  GNX18_RS12500 (GNX18_12485) pilM 3064939..3066015 (-) 1077 WP_166218514.1 pilus assembly protein PilM Machinery gene
  GNX18_RS12505 (GNX18_12490) - 3066171..3068867 (+) 2697 WP_166218516.1 penicillin-binding protein 1A -
  GNX18_RS12510 (GNX18_12495) - 3068965..3069450 (-) 486 WP_138235208.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38973.27 Da        Isoelectric Point: 4.3982

>NTDB_id=409604 GNX18_RS12500 WP_166218514.1 3064939..3066015(-) (pilM) [Microbulbifer sp. SH-1]
MGMLPFLNKGPKAMLGLDISSTSVKLLELSRSGDKYRVESYATEPLPPNAVVDKNINDVGAIAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLEMPADLSDDALEAQIALEADQYIPYPLDEVNLDFEVQGPSERGEGQVEVLLAACRSENVEQRVSAL
TQAELQPGVVDVEAYAIERAYTLMENQFPAQEQLVVAVVDIGATMSALSVMVDGRIVYTREQLFGGRQLTDEIQRRYGLS
SEEAALAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMEGLAELVGQKLNKPTLV
ANPFRDMSVASRVNRQSLAGEAPSLMIAAGLAMREREF

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=409604 GNX18_RS12500 WP_166218514.1 3064939..3066015(-) (pilM) [Microbulbifer sp. SH-1]
ATGGGGATGCTCCCTTTTCTCAACAAGGGCCCGAAAGCCATGCTTGGGCTCGATATTAGCTCCACCTCGGTAAAGTTGCT
TGAACTGAGTCGCAGCGGCGACAAATACCGCGTGGAAAGCTATGCAACCGAGCCTCTGCCTCCGAATGCGGTAGTGGATA
AGAATATCAATGACGTGGGTGCAATCGCCGAGGCCATTCGCAAGGTGGTGCGCCGCTCCAAGACCCGCCTGCGCCAGGCT
GCAGTGGCGGTATCGGGCTCCGCGGTCATTACCAAGACCCTGGAAATGCCGGCGGACCTCTCCGACGACGCGCTGGAAGC
GCAGATTGCGCTGGAAGCCGACCAGTACATTCCCTACCCGCTGGATGAAGTGAATCTCGATTTCGAAGTACAGGGTCCTT
CCGAAAGAGGCGAAGGCCAGGTCGAGGTATTGCTGGCGGCATGCCGCAGTGAAAACGTTGAGCAGCGTGTTTCCGCGCTG
ACCCAGGCGGAGCTGCAGCCGGGCGTGGTGGATGTGGAGGCCTACGCCATCGAGCGCGCCTACACCCTGATGGAAAACCA
GTTCCCGGCGCAGGAACAGCTGGTGGTGGCGGTTGTAGATATCGGCGCCACCATGAGTGCGCTGTCGGTGATGGTCGATG
GCCGTATCGTCTACACCCGCGAGCAGTTGTTCGGCGGGCGCCAGCTCACCGACGAGATCCAGCGGCGCTACGGTCTGTCG
TCGGAAGAGGCTGCATTGGCCAAGCGCCAGGGCGGTAGCGGCCTGCCGGATGATTACTATCCCGAAGTTCTGGAGCCGTT
CCGCGACGCGGTGGTGCAGCAGGTCACCCGCTCCCTGCAGTTTTTCTATTCCTCCACCTCCTACAGCGATGTGGATTACA
TCCTCCTCGGTGGCGGTGTTGCCGCGATGGAGGGTCTCGCCGAACTGGTGGGGCAGAAACTGAACAAACCCACTCTGGTG
GCCAATCCCTTCCGGGATATGAGTGTCGCGTCCCGGGTCAATCGCCAGTCGCTGGCCGGCGAGGCGCCGTCGCTGATGAT
CGCCGCCGGGCTCGCGATGCGCGAGAGGGAATTCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8DFR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.841

98.324

0.52
  comM Acinetobacter nosocomialis M2

52.841

98.324

0.52
  comM Acinetobacter baylyi ADP1

48.295

98.324

0.475
  pilM Legionella pneumophila strain ERS1305867

44.663

99.441

0.444

Multiple sequence alignment