Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   METME_RS01360 Genome accession   NC_015572
Coordinates   307779..308840 (+) Length   353 a.a.
NCBI ID   WP_013817004.1    Uniprot ID   F9ZZS2
Organism   Methylomonas methanica MC09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 302779..313840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS01350 (Metme_0279) - 303488..304843 (+) 1356 WP_013817001.1 AmpG family muropeptide MFS transporter -
  METME_RS23785 (Metme_0280) rpmE 304937..305137 (+) 201 WP_013817002.1 50S ribosomal protein L31 -
  METME_RS01355 (Metme_0281) - 305200..307536 (-) 2337 WP_041363692.1 penicillin-binding protein 1A -
  METME_RS01360 (Metme_0282) pilM 307779..308840 (+) 1062 WP_013817004.1 pilus assembly protein PilM Machinery gene
  METME_RS01365 (Metme_0283) - 308840..309412 (+) 573 WP_013817005.1 PilN domain-containing protein -
  METME_RS01370 (Metme_0284) - 309409..310041 (+) 633 WP_013817006.1 type 4a pilus biogenesis protein PilO -
  METME_RS01375 (Metme_0285) - 310038..310595 (+) 558 WP_041363693.1 pilus assembly protein PilP -
  METME_RS01380 (Metme_0286) pilQ 310609..312873 (+) 2265 WP_013817008.1 type IV pilus secretin family protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38348.97 Da        Isoelectric Point: 4.7465

>NTDB_id=40915 METME_RS01360 WP_013817004.1 307779..308840(+) (pilM) [Methylomonas methanica MC09]
MSWFNRNQSALLGIDISTAAVKLLELSRTGARYKVESYAVAPLPQDAIIDKNITNVELIGNAIKAAVKQSGSRAKRACVA
VAGSSVMTKIISMPASLSDAEMEEQIMVEADQYIPYSLDEVNLDFDVQGVSKNNPEMLDVLLAASRRENIEDRIEALAFA
GLKATIVDVEAFAMENAFALLVDQLPERVENKTVAIADIGATMTSLNILHEGKTIYTREQGFGGRQLTEEIQRRYGLSYE
EAGLAKRHGGLPDNYIPDVLEPFKKAMLQQIARSLQFFVSSSANRGVDALILAGGCASIAGLEKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=40915 METME_RS01360 WP_013817004.1 307779..308840(+) (pilM) [Methylomonas methanica MC09]
ATGAGCTGGTTTAACAGGAATCAGTCTGCTTTGCTTGGCATTGATATCAGTACCGCTGCGGTTAAATTGCTGGAGTTAAG
TCGAACCGGTGCCCGCTACAAGGTTGAAAGCTATGCGGTGGCACCTCTGCCGCAAGATGCGATTATTGATAAAAATATTA
CCAATGTGGAACTGATCGGTAACGCGATAAAGGCTGCGGTCAAACAATCCGGTAGTCGGGCAAAGCGGGCGTGCGTCGCG
GTCGCCGGGTCGTCGGTGATGACTAAAATTATATCCATGCCGGCCTCGCTGAGCGATGCTGAAATGGAAGAACAGATTAT
GGTGGAAGCCGATCAATACATACCGTATTCGCTGGACGAAGTTAATCTCGATTTCGATGTGCAAGGTGTTTCGAAAAACA
ATCCGGAGATGTTGGATGTGTTGTTGGCGGCTTCGCGGCGGGAAAACATTGAAGATCGCATTGAGGCTTTGGCTTTCGCC
GGCTTGAAAGCCACGATTGTGGATGTAGAGGCGTTTGCCATGGAAAATGCTTTTGCCCTGTTAGTCGATCAGTTGCCTGA
ACGCGTTGAAAACAAAACCGTGGCAATTGCCGATATCGGCGCCACCATGACTTCGTTGAATATCTTGCATGAAGGTAAAA
CCATTTACACGCGCGAGCAAGGTTTTGGTGGGCGACAGCTTACCGAGGAGATCCAGCGGCGCTACGGTTTGTCTTATGAA
GAGGCCGGACTCGCCAAACGGCATGGCGGCTTACCCGATAACTATATCCCCGATGTGCTGGAACCTTTTAAAAAGGCCAT
GTTGCAGCAGATTGCACGGTCATTGCAGTTTTTTGTGTCCTCCAGCGCGAACAGAGGTGTCGATGCCTTAATTTTGGCGG
GGGGATGCGCGTCCATCGCCGGCTTGGAAAAGTTAGTCGAGCGCGACTTGGGTATTCCTTCTTATATCGCTAACCCGTTT
ATCAATATGGCGCTTTCCAACCGCGTCAAGCCGCAGAGTTTAAGCAACGATACGCCGGCGATGATGATAGCCTGCGGTTT
GGCGTTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F9ZZS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.292

100

0.493
  comM Acinetobacter nosocomialis M2

49.292

100

0.493
  comM Acinetobacter baylyi ADP1

48.159

100

0.482
  pilM Legionella pneumophila strain ERS1305867

41.926

100

0.419

Multiple sequence alignment