Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX95_RS08800 Genome accession   NZ_CP046817
Coordinates   1913457..1914473 (+) Length   338 a.a.
NCBI ID   WP_055032060.1    Uniprot ID   -
Organism   Vibrio metoecus strain 06-2478     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1908457..1919473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX95_RS08785 - 1908977..1909708 (-) 732 WP_055029202.1 glutathione peroxidase -
  GPX95_RS08790 oxyR 1909849..1910742 (+) 894 WP_055032061.1 DNA-binding transcriptional regulator OxyR -
  GPX95_RS08795 - 1910838..1913315 (-) 2478 WP_055027426.1 penicillin-binding protein 1A -
  GPX95_RS08800 pilM 1913457..1914473 (+) 1017 WP_055032060.1 type IV pilus assembly protein PilM Machinery gene
  GPX95_RS08805 pilN 1914463..1915041 (+) 579 WP_055032059.1 PilN domain-containing protein Machinery gene
  GPX95_RS08810 pilO 1915034..1915624 (+) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX95_RS08815 pilP 1915614..1916132 (+) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  GPX95_RS08820 pilQ 1916158..1917873 (+) 1716 WP_199250716.1 type IV pilus secretin PilQ Machinery gene
  GPX95_RS08825 aroK 1918060..1918584 (+) 525 WP_000818614.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37597.42 Da        Isoelectric Point: 7.2629

>NTDB_id=408319 GPX95_RS08800 WP_055032060.1 1913457..1914473(+) (pilM) [Vibrio metoecus strain 06-2478]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSQKVAIAIPDNA
VISKVLQIDSDLELREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNLARSSTTTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLASRAYRRADWMLIDIGHTQSSLCLDFPDKMPFYKDVPLGTRLVDLDSASPSILNPPQLHDSAQR
FINELVDKVARQIQLFTSVHGAQSLNGLWLSGGGAAMEGLPEALSQRLSLPCEVLNPFALFKMQVSKRKRQVVDGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=408319 GPX95_RS08800 WP_055032060.1 1913457..1914473(+) (pilM) [Vibrio metoecus strain 06-2478]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCCGTGGTGCTCAAACCTATGGGCGACAC
CTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTACCCCTATTTAGTCAGAAAGTCGCGATTGCAATCCCGGATAACGCG
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCTGCGAGAGCAGGAGTTCGCTATTTATCAGGCTTTTTCCCA
CCAATCTCCATTTCCCGTTGAAGATCTTAGCCTTGATTTCGTCAAAGTGGCGGAAAAAAATCTCGCACGCAGTAGTACGA
CGACGTTTCAAGTCTATGCCACCAAGAAAGAAGTGGTTGAAAGCCGCTTACAAGCCAGTAAAAAAGCAGGTTTTGAGCCG
ATCTTAATGGATGTGCAAGTGCACAGTTTGTTGCATCTATGGCAACTCGCTAGCCGCGCGTATCGCCGTGCGGACTGGAT
GCTGATTGATATTGGACACACACAAAGCTCTTTGTGTTTGGACTTTCCAGACAAAATGCCTTTTTACAAAGATGTGCCAC
TAGGAACTCGATTGGTCGATTTAGACTCTGCCAGCCCATCCATACTCAATCCGCCTCAATTGCACGACTCGGCTCAACGT
TTTATCAATGAATTGGTGGATAAAGTGGCGCGTCAAATCCAACTGTTTACGTCGGTGCATGGAGCACAAAGCCTCAATGG
TTTGTGGCTATCAGGCGGTGGTGCGGCGATGGAAGGATTGCCAGAAGCGTTGTCCCAGCGTTTATCTTTGCCTTGTGAAG
TGTTGAATCCTTTTGCGTTGTTCAAAATGCAGGTCTCAAAACGTAAACGCCAAGTCGTTGATGGCCAACGTTTTAGCACT
GCTGCAGGGCTCGCTTTGCGTGGTTTAGCATGGTTGGAGAGTGAGCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-200)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

84.911

100

0.849
  pilM Vibrio campbellii strain DS40M4

49.415

100

0.5

Multiple sequence alignment