Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPX95_RS08820 Genome accession   NZ_CP046817
Coordinates   1916158..1917873 (+) Length   571 a.a.
NCBI ID   WP_199250716.1    Uniprot ID   -
Organism   Vibrio metoecus strain 06-2478     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1911158..1922873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX95_RS08800 pilM 1913457..1914473 (+) 1017 WP_055032060.1 type IV pilus assembly protein PilM Machinery gene
  GPX95_RS08805 pilN 1914463..1915041 (+) 579 WP_055032059.1 PilN domain-containing protein Machinery gene
  GPX95_RS08810 pilO 1915034..1915624 (+) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX95_RS08815 pilP 1915614..1916132 (+) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  GPX95_RS08820 pilQ 1916158..1917873 (+) 1716 WP_199250716.1 type IV pilus secretin PilQ Machinery gene
  GPX95_RS08825 aroK 1918060..1918584 (+) 525 WP_000818614.1 shikimate kinase AroK -
  GPX95_RS08830 aroB 1918612..1919697 (+) 1086 WP_055030904.1 3-dehydroquinate synthase -
  GPX95_RS08835 - 1919751..1921229 (+) 1479 WP_095475744.1 AAA family ATPase -
  GPX95_RS08840 - 1921299..1922132 (+) 834 WP_000744677.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62367.41 Da        Isoelectric Point: 4.6569

>NTDB_id=408323 GPX95_RS08820 WP_199250716.1 1916158..1917873(+) (pilQ) [Vibrio metoecus strain 06-2478]
MRNGLKTYVAQAWLTLWVGLVLCATSVAHSAESASTNQLQNIDFRVNKDKAAVLIVELASPSVVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSIEVFRKEPSTQLIATIDGEFKHDYSLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKDELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGSIESNLWQQGLLADDEFPVDQFLNVNLASASANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=408323 GPX95_RS08820 WP_199250716.1 1916158..1917873(+) (pilQ) [Vibrio metoecus strain 06-2478]
ATGAGAAATGGATTAAAAACTTATGTCGCACAGGCTTGGCTGACCTTATGGGTTGGTTTGGTGCTTTGTGCTACTTCCGT
AGCGCATAGCGCGGAGTCGGCTTCTACCAATCAACTGCAAAATATTGATTTTCGGGTCAATAAAGACAAAGCCGCAGTGC
TGATTGTGGAGTTAGCTTCCCCCTCTGTTGTCGTGGATGTTCAAAAAGTCCAAGAAGGGCTGAGTATTGAGCTGCTAAAA
ACGGATGTGGCAGATGACAAGCTTTACTTATTGGATGTAAAGGACTTTTCCACTCCTGTTCAAAGTATAGAAGTGTTCCG
CAAAGAACCTAGCACTCAGTTGATTGCGACCATTGATGGTGAGTTTAAACATGACTACAGCTTGAAAGGAAAATACTTGG
AGGTGGTGATCAGTAAGCTTAAAGCAGATGAAAAACCACAGCCGAAAAGTATTCTAGAGAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCTGTGCGTAACGTACTGCAGCTAATTGCCGATTATAACGGCTTCAACTTAGTGGTATCGGA
CTCGGTGGTCGGTAACCTGACGTTACGCTTGGATGGGGTGCCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAGG
GCTTGGATAAGCGTGTTGATGGTAACGTTATTCTCATCGCACCCAAAGACGAGTTGGATCTACGAGAAAAGCAGGCTTTA
GAGAAAGCGCGTTTAGCGGAAGAACTGGGTGATTTAAAATCAGAAATCATCAAAATCAATTTTGCTAAAGCTTCAGATAT
CGCCGCCATGATTGGCGGAGAAGGCAACGTCAATATGCTCTCTGAGCGGGGATCGATCAGCATTGATGAGCGTACTAACT
CGCTACTGATCCGAGAGTTACCCGATAATATTGCCGTGATCCGCGAGATCATCGAATCGCTGGATATTCCGGTCAAGCAA
GTGCAAATCGAAGCACGTATAGTTACTGTCAAAGAGGGTAACTTGGATGAGCTTGGTGTGCGTTGGGGGGTGATGTCAAC
CAACGGTAGCCATTCGGTTGGCGGAAGTATTGAAAGTAACTTATGGCAACAAGGACTTTTGGCTGACGACGAATTTCCGG
TCGATCAGTTCTTAAATGTGAACTTGGCGTCAGCATCAGCGAACGCTTCCTCTATCGCTTTCCAAGTGGCTAAGCTTGGT
TCCGATACCTTATTGGATTTAGAGCTCTCAGCACTCCAAAACGAGTCGAAAGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACCACTAATAAACAACCTGCGTACATCGAGCAGGGTACGGAAATCCCATACCTTGAGTCATCATCGAGTGGAGCGTCTA
CCGTATCGTTTAAAAAAGCGGTATTGAGCTTGAAAGTTACCCCGCAAATTACGCCGGATAACCGCTTGGTGCTGGATTTG
AGTGTGACTCAAGACCGCCGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACACAAGTATTAGTGAATAATGGAGAAACAGTCGTATTAGGTGGCATTTTCCAGCACAGCATCAATAATTCAGTGGATA
AGGTGCCGCTGCTTGGAGACCTGCCAGTTTTAGGTGCGCTATTTAGACGAACGTACGAGCAAATGGGCAAAAGTGAGCTG
TTGATTTTCGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.096

100

0.951
  pilQ Vibrio cholerae strain A1552

95.096

100

0.951
  pilQ Vibrio campbellii strain DS40M4

78.909

96.322

0.76
  pilQ Legionella pneumophila strain Lp02

42.989

94.921

0.408
  pilQ Legionella pneumophila strain ERS1305867

42.804

94.921

0.406
  comQ Acinetobacter baylyi ADP1

39.619

100

0.401
  pilQ Acinetobacter baumannii D1279779

38.055

100

0.391
  pilQ Acinetobacter baumannii strain A118

38.055

100

0.391
  pilQ Pseudomonas aeruginosa PAK

38.879

96.848

0.377

Multiple sequence alignment