Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY18_RS16345 Genome accession   NZ_CP046800
Coordinates   2003742..2004326 (+) Length   194 a.a.
NCBI ID   WP_158147077.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2012V-1235     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1998742..2009326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY18_RS16330 - 1999542..2002022 (-) 2481 WP_158147075.1 PBP1A family penicillin-binding protein -
  GPY18_RS16335 pilM 2002165..2003169 (+) 1005 WP_024374095.1 type IV pilus assembly protein PilM Machinery gene
  GPY18_RS16340 pilN 2003159..2003749 (+) 591 WP_158147076.1 PilN domain-containing protein Machinery gene
  GPY18_RS16345 pilO 2003742..2004326 (+) 585 WP_158147077.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY18_RS16350 pilP 2004316..2004828 (+) 513 WP_158147078.1 pilus assembly protein PilP Machinery gene
  GPY18_RS16355 pilQ 2004853..2006589 (+) 1737 WP_233927180.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY18_RS16360 aroK 2006777..2007295 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY18_RS16365 aroB 2007323..2008408 (+) 1086 WP_020329448.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22493.86 Da        Isoelectric Point: 4.8022

>NTDB_id=408020 GPY18_RS16345 WP_158147077.1 2003742..2004326(+) (pilO) [Vibrio fluvialis strain 2012V-1235]
MANLQELEEIAEWPLLPQLAVLLLLVLALQGAGYWLYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDELA
ERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGEKQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRIINF
DDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 585 bp        

>NTDB_id=408020 GPY18_RS16345 WP_158147077.1 2003742..2004326(+) (pilO) [Vibrio fluvialis strain 2012V-1235]
ATGGCTAACTTGCAGGAACTGGAAGAAATCGCTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCTGGTTCT
GGCACTGCAAGGTGCGGGCTATTGGTTATATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGGAACAAA
CGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAACTGGCA
GAACGGTATGACTTCCTGCTGCGTCAACTGCCAGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGAGCTGGG
GCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAAGCAGAATCAGGAGTTCCTCTATCGTCTGCCACTCA
ATATCGAACTGACCGGCAATTATCACAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATCAACTTT
GACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCAGTTCAA
ACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(45-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

78.571

100

0.794
  pilO Vibrio campbellii strain DS40M4

68.421

97.938

0.67

Multiple sequence alignment