Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY18_RS16355 Genome accession   NZ_CP046800
Coordinates   2004853..2006589 (+) Length   578 a.a.
NCBI ID   WP_233927180.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2012V-1235     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1999853..2011589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY18_RS16335 pilM 2002165..2003169 (+) 1005 WP_024374095.1 type IV pilus assembly protein PilM Machinery gene
  GPY18_RS16340 pilN 2003159..2003749 (+) 591 WP_158147076.1 PilN domain-containing protein Machinery gene
  GPY18_RS16345 pilO 2003742..2004326 (+) 585 WP_158147077.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY18_RS16350 pilP 2004316..2004828 (+) 513 WP_158147078.1 pilus assembly protein PilP Machinery gene
  GPY18_RS16355 pilQ 2004853..2006589 (+) 1737 WP_233927180.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY18_RS16360 aroK 2006777..2007295 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY18_RS16365 aroB 2007323..2008408 (+) 1086 WP_020329448.1 3-dehydroquinate synthase -
  GPY18_RS16370 - 2008454..2009962 (+) 1509 WP_158147079.1 AAA family ATPase -
  GPY18_RS16375 - 2010057..2010887 (+) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62424.38 Da        Isoelectric Point: 4.5373

>NTDB_id=408022 GPY18_RS16355 WP_233927180.1 2004853..2006589(+) (pilQ) [Vibrio fluvialis strain 2012V-1235]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGASGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=408022 GPY18_RS16355 WP_233927180.1 2004853..2006589(+) (pilQ) [Vibrio fluvialis strain 2012V-1235]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTGGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCCGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCCTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGCTGGGGTTTCAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGCAATAATAGTGGCG
CTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAATGTGAACCTCGCCGCTACCTCCGCCAATGCATCCAGTATTGCG
TTTCAGGTCGCCAAGCTCGGCGCAGATACCTTGCTCGATCTTGAACTATCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACCACCAACAAGCAGCCGGCGTATATTGAACAGGGTACTGAGATTCCGTATCTGGAAT
CCTCGTCCAGTGGAGCCACCAGTGTGGCATTTAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCACAAATCACCCCCGAT
AACCGTCTGGTGCTGGATTTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGCACTGGCGAGGCGGTGGC
GATCAACACGCAGCGTATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCCGTGTTAGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.693

100

0.818
  pilQ Vibrio cholerae strain A1552

81.693

100

0.818
  pilQ Vibrio campbellii strain DS40M4

79.235

94.983

0.753
  pilQ Legionella pneumophila strain Lp02

42.545

95.156

0.405
  pilQ Legionella pneumophila strain ERS1305867

42.545

95.156

0.405
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382

Multiple sequence alignment