Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY18_RS16335 Genome accession   NZ_CP046800
Coordinates   2002165..2003169 (+) Length   334 a.a.
NCBI ID   WP_024374095.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2012V-1235     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1997165..2008169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY18_RS16315 - 1997315..1997578 (-) 264 WP_020429299.1 DUF3624 domain-containing protein -
  GPY18_RS16320 - 1997707..1998441 (-) 735 WP_061057153.1 glutathione peroxidase -
  GPY18_RS16325 oxyR 1998585..1999481 (+) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GPY18_RS16330 - 1999542..2002022 (-) 2481 WP_158147075.1 PBP1A family penicillin-binding protein -
  GPY18_RS16335 pilM 2002165..2003169 (+) 1005 WP_024374095.1 type IV pilus assembly protein PilM Machinery gene
  GPY18_RS16340 pilN 2003159..2003749 (+) 591 WP_158147076.1 PilN domain-containing protein Machinery gene
  GPY18_RS16345 pilO 2003742..2004326 (+) 585 WP_158147077.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY18_RS16350 pilP 2004316..2004828 (+) 513 WP_158147078.1 pilus assembly protein PilP Machinery gene
  GPY18_RS16355 pilQ 2004853..2006589 (+) 1737 WP_233927180.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY18_RS16360 aroK 2006777..2007295 (+) 519 WP_004728569.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36849.18 Da        Isoelectric Point: 6.6961

>NTDB_id=408018 GPY18_RS16335 WP_024374095.1 2002165..2003169(+) (pilM) [Vibrio fluvialis strain 2012V-1235]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFAVYQAFSHQSPLPIEELSLDFVVTGEKTLGRSSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTDWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTSPQAHFIQE
LVERLARQIQLFLSLQGHQPLAGIWLSGGGAATEGLAQAISERLALPCELFNPLAEFTCRASLRRQPQADLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=408018 GPY18_RS16335 WP_024374095.1 2002165..2003169(+) (pilM) [Vibrio fluvialis strain 2012V-1235]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCAGCGAACAAGAGTTCGCGGTCTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTTACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCACCGATTGGAT
GCTGGTCGATGTCGGGTTCAGCCAAACCTCATTGTGCATCGACTTTGCCGATAAAACGCCGTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCTACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAG
TTGGTTGAGCGTCTGGCGCGCCAGATCCAGCTGTTTCTTTCTCTGCAAGGCCATCAACCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGCTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCGCCAGCCTCAGGCTGATTTGCAGCGTTTCAGTACTGCGGCCGGCTTG
GCGCTGCGCGGGCTGAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

65.385

100

0.662
  pilM Vibrio campbellii strain DS40M4

48.406

100

0.5

Multiple sequence alignment