Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPW70_RS06370 Genome accession   NZ_CP046740
Coordinates   306746..308461 (+) Length   571 a.a.
NCBI ID   WP_032472752.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3566-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 301746..313461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW70_RS06350 pilM 304039..305055 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  GPW70_RS06355 pilN 305045..305629 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  GPW70_RS06360 pilO 305622..306212 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW70_RS06365 pilP 306202..306720 (+) 519 WP_032472751.1 pilus assembly protein PilP Machinery gene
  GPW70_RS06370 pilQ 306746..308461 (+) 1716 WP_032472752.1 type IV pilus secretin PilQ Machinery gene
  GPW70_RS06375 aroK 308648..309172 (+) 525 WP_000818616.1 shikimate kinase AroK -
  GPW70_RS06380 aroB 309200..310285 (+) 1086 WP_032472753.1 3-dehydroquinate synthase -
  GPW70_RS06385 - 310340..311815 (+) 1476 WP_001888631.1 AAA family ATPase -
  GPW70_RS06390 - 311885..312718 (+) 834 WP_001888629.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62326.46 Da        Isoelectric Point: 4.6094

>NTDB_id=406908 GPW70_RS06370 WP_032472752.1 306746..308461(+) (pilQ) [Vibrio cholerae strain 3566-06]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVDSVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=406908 GPW70_RS06370 WP_032472752.1 306746..308461(+) (pilQ) [Vibrio cholerae strain 3566-06]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGATAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCTGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTCCTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTCAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGCCGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTAGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTGGACTTG
AGCGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGCACTGGCGAAGCGGTTTCTATTGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.825

100

0.998
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.825

100

0.998
  pilQ Vibrio campbellii strain DS40M4

78.364

96.322

0.755
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment