Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HZT35_RS01240 Genome accession   NZ_CP058682
Coordinates   277014..277778 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain MBT-5     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 272014..282778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZT35_RS01230 (HZT35_19075) nikR 275804..276205 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HZT35_RS01235 (HZT35_19080) nikE 276211..277017 (-) 807 WP_000173630.1 nickel import ATP-binding protein NikE -
  HZT35_RS01240 (HZT35_19085) amiE 277014..277778 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  HZT35_RS01245 (HZT35_19090) nikC 277778..278611 (-) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  HZT35_RS01250 (HZT35_19095) nikB 278608..279552 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  HZT35_RS01255 (HZT35_19100) nikA 279552..281126 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  HZT35_RS01260 (HZT35_19105) acpT 281237..281824 (-) 588 WP_000285771.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=406409 HZT35_RS01240 WP_001136229.1 277014..277778(-) (amiE) [Escherichia coli strain MBT-5]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=406409 HZT35_RS01240 WP_001136229.1 277014..277778(-) (amiE) [Escherichia coli strain MBT-5]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment