Detailed information NTG0405573

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GNH96_RS01755
• Genome accession: NZ_CP046565
• Coordinates: 375196..376257 (-)
• Length: 353 a.a.
• NCBI ID: WP_169601709.1
• Uniprot ID: A0A858Q4P5
• Organism: Methylococcus geothermalis strain IM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 370196..381257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GNH96_RS01730 (GNH96_01725)	aroK	370622..371161 (-)	540	WP_169601699.1	shikimate kinase AroK	-
GNH96_RS01735 (GNH96_01730)	pilQ	371226..373412 (-)	2187	WP_169601701.1	type IV pilus secretin PilQ	-
GNH96_RS01740 (GNH96_01735)	-	373399..373950 (-)	552	WP_169601703.1	pilus assembly protein PilP	-
GNH96_RS01745 (GNH96_01740)	-	373950..374594 (-)	645	WP_169601705.1	type 4a pilus biogenesis protein PilO	-
GNH96_RS01750 (GNH96_01745)	-	374591..375196 (-)	606	WP_169601707.1	PilN domain-containing protein	-
GNH96_RS01755 (GNH96_01750)	pilM	375196..376257 (-)	1062	WP_169601709.1	pilus assembly protein PilM	Machinery gene
GNH96_RS01760 (GNH96_01755)	-	376591..379041 (+)	2451	WP_228719970.1	penicillin-binding protein 1A	-
GNH96_RS01765 (GNH96_01760)	-	379058..379654 (+)	597	WP_228719971.1	hypothetical protein	-
GNH96_RS01770 (GNH96_01765)	rpmE	379712..379915 (-)	204	WP_169601713.1	50S ribosomal protein L31	-
GNH96_RS01775 (GNH96_01770)	-	380058..380831 (+)	774	WP_169601715.1	exodeoxyribonuclease III	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38497.86 Da
• Isoelectric Point: 4.7370

>NTDB_id=405573 GNH96_RS01755 WP_169601709.1 375196..376257(-) (pilM) [Methylococcus geothermalis strain IM1]
MWGFGRKKPLLLGIDISAAAVKLLELSRKDGGYQVESYGVVPLPRNTVVDNTIAEPDNVSAAVRAVVKQSGTSLKHAVVA
VPGSVVITKRITLPANLEGDELEAQIELEADQYIPHAREEVSLDFEMLGKSAKNPDLVEVLLVATRRENVEDRVSVLENA
GLGVEIVDVESYAIEHAFELIKDQLPPDARDRAVAVADVGAMVTTLNVIHGGSIIYTREQGFGGMQLTDEIQRRYGLTYE
EAGLAKKEGSLPGNYAEEVLEPFKHALVQQISRSFQFYLSSTVQRGFDAVVLAGGCAMIPEIDRYVEAALRVPTVVANPF
RNMSFSGRVHQERLNYDSSALMVACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=405573 GNH96_RS01755 WP_169601709.1 375196..376257(-) (pilM) [Methylococcus geothermalis strain IM1]
ATGTGGGGCTTCGGACGGAAGAAACCGCTCCTGCTCGGGATTGACATCAGCGCGGCGGCGGTCAAGCTGCTGGAGCTGAG
CAGGAAGGATGGCGGCTACCAGGTGGAAAGCTACGGTGTCGTGCCGCTGCCCCGGAACACCGTGGTCGACAACACGATCG
CCGAGCCCGACAACGTATCGGCGGCGGTGCGCGCCGTGGTCAAGCAGTCGGGTACCTCGCTCAAGCACGCGGTGGTCGCG
GTGCCGGGATCGGTGGTCATCACCAAGCGCATCACCCTGCCGGCCAACCTGGAAGGCGACGAGCTGGAGGCCCAGATCGA
ACTGGAGGCGGACCAGTACATTCCTCATGCGAGGGAAGAAGTCAGCCTCGATTTCGAGATGCTGGGGAAAAGCGCGAAAA
ATCCCGACCTGGTGGAAGTGCTTCTGGTGGCTACGCGCCGGGAAAATGTCGAAGACCGGGTTTCGGTGCTGGAGAACGCG
GGCCTCGGTGTCGAGATCGTCGATGTGGAGTCCTATGCCATCGAGCACGCCTTCGAACTGATCAAGGACCAACTCCCGCC
GGATGCACGCGACCGGGCGGTGGCCGTGGCCGATGTGGGGGCGATGGTCACCACGCTCAACGTCATTCATGGCGGTTCCA
TCATTTACACCCGTGAGCAGGGCTTCGGCGGCATGCAACTGACCGACGAGATTCAGCGCCGTTACGGCCTGACCTACGAG
GAGGCCGGCCTGGCGAAGAAGGAAGGGAGCCTCCCCGGCAACTATGCCGAGGAAGTGCTGGAGCCGTTCAAGCATGCCCT
CGTGCAGCAGATCAGCCGCTCGTTTCAGTTCTATCTGTCGTCCACGGTTCAGCGCGGTTTCGATGCGGTGGTGCTGGCCG
GAGGCTGCGCCATGATTCCGGAGATCGATCGTTATGTGGAGGCGGCATTGCGGGTGCCCACCGTGGTCGCGAACCCTTTC
CGTAACATGAGTTTTTCCGGGCGAGTGCATCAGGAACGCCTGAACTACGATTCCTCGGCCCTCATGGTGGCCTGCGGTCT
GGCCTTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A858Q4P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.312	97.167	0.499
comM	Acinetobacter nosocomialis M2	50.729	97.167	0.493
comM	Acinetobacter baylyi ADP1	48.286	99.15	0.479
pilM	Legionella pneumophila strain ERS1305867	43.023	97.45	0.419