Detailed information NTG0405404

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GOQ05_RS04575
• Genome accession: NZ_CP046552
• Coordinates: 936787..937905 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 19WIARLN021_full
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 931787..942905

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GOQ05_RS04545 (GOQ05_04515)	-	932616..933464 (+)	849	WP_304506014.1	class II glutamine amidotransferase	-
GOQ05_RS04550 (GOQ05_04520)	-	933717..934172 (+)	456	WP_000782976.1	bacteriohemerythrin	-
GOQ05_RS04555 (GOQ05_04525)	-	934325..935392 (+)	1068	WP_029777899.1	membrane protein	-
GOQ05_RS04560 (GOQ05_04530)	-	935392..935715 (+)	324	WP_000401389.1	RnfH family protein	-
GOQ05_RS04565 (GOQ05_04535)	-	935770..936168 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
GOQ05_RS04570 (GOQ05_04540)	fur	936280..936717 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
GOQ05_RS04575 (GOQ05_04545)	pilU	936787..937905 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GOQ05_RS04580 (GOQ05_04550)	pilT	937933..938970 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
GOQ05_RS04585 (GOQ05_04555)	-	939097..939789 (+)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=405404 GOQ05_RS04575 WP_000347034.1 936787..937905(-) (pilU) [Acinetobacter baumannii strain 19WIARLN021_full]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=405404 GOQ05_RS04575 WP_000347034.1 936787..937905(-) (pilU) [Acinetobacter baumannii strain 19WIARLN021_full]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACTAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39