Detailed information NTG0405363

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GOQ08_RS14590
• Genome accession: NZ_CP046549
• Coordinates: 3042591..3043709 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 19WIARLN022_full
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3037591..3048709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GOQ08_RS14580 (GOQ08_14450)	-	3040707..3041399 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
GOQ08_RS14585 (GOQ08_14455)	pilT	3041526..3042563 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
GOQ08_RS14590 (GOQ08_14460)	pilU	3042591..3043709 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GOQ08_RS14595 (GOQ08_14465)	fur	3043779..3044216 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
GOQ08_RS14600 (GOQ08_14470)	-	3044328..3044726 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
GOQ08_RS14605 (GOQ08_14475)	-	3044781..3045104 (-)	324	WP_000401389.1	RnfH family protein	-
GOQ08_RS14610 (GOQ08_14480)	-	3045104..3046171 (-)	1068	WP_029777899.1	membrane protein	-
GOQ08_RS14615 (GOQ08_14485)	-	3046324..3046779 (-)	456	WP_000782976.1	bacteriohemerythrin	-
GOQ08_RS14620 (GOQ08_14490)	-	3047032..3047880 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=405363 GOQ08_RS14590 WP_000347034.1 3042591..3043709(+) (pilU) [Acinetobacter baumannii strain 19WIARLN022_full]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=405363 GOQ08_RS14590 WP_000347034.1 3042591..3043709(+) (pilU) [Acinetobacter baumannii strain 19WIARLN022_full]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACTAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39