Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   GM698_RS01965 Genome accession   NZ_CP046532
Coordinates   440135..441448 (+) Length   437 a.a.
NCBI ID   WP_176812332.1    Uniprot ID   -
Organism   Mannheimia sp. ZY171111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435135..446448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM698_RS01940 (GM698_01930) - 435299..437848 (-) 2550 WP_176812337.1 penicillin-binding protein 1A -
  GM698_RS01945 (GM698_01935) - 437975..438682 (+) 708 WP_209289578.1 competence protein ComA -
  GM698_RS01950 (GM698_01940) - 438658..439179 (+) 522 WP_176808053.1 hypothetical protein -
  GM698_RS01955 (GM698_01945) - 439181..439714 (+) 534 WP_176808052.1 hypothetical protein -
  GM698_RS01960 (GM698_01950) - 439716..440126 (+) 411 WP_176808051.1 hypothetical protein -
  GM698_RS01965 (GM698_01955) comE 440135..441448 (+) 1314 WP_176812332.1 type IV pilus secretin PilQ Machinery gene
  GM698_RS01970 (GM698_01960) nusB 441528..441941 (+) 414 WP_176812331.1 transcription antitermination factor NusB -
  GM698_RS01975 (GM698_01965) thiL 442005..442985 (+) 981 WP_176812330.1 thiamine-phosphate kinase -
  GM698_RS01980 (GM698_01970) - 442991..443473 (+) 483 WP_176808048.1 phosphatidylglycerophosphatase A -
  GM698_RS01985 (GM698_01975) - 443467..444093 (+) 627 WP_159629280.1 LysE family transporter -
  GM698_RS01990 (GM698_01980) ruvA 444202..444804 (+) 603 WP_209289579.1 Holliday junction branch migration protein RuvA -
  GM698_RS01995 (GM698_01985) ruvB 444815..445819 (+) 1005 WP_176808046.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48427.66 Da        Isoelectric Point: 8.9535

>NTDB_id=405171 GM698_RS01965 WP_176812332.1 440135..441448(+) (comE) [Mannheimia sp. ZY171111]
MFRIFLFLFISSSLAFANTISLAVKNAPTATILTYLSEEIGKNIVLDDNIQTKSTLRLENKSIDDIFKVISTMNKLSLHQ
EGDVIYISKKEEKLTDLKTIPIAHLQNNIENTPLVTTPKLITKTIKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSTKSLQHIEKLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSKGADHYKFGGRLESNGLSNVANNLNVS
FPVTNGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNKKSEATNVEFKEAVLG
LEVIPHLSTENQILLDLIVTQNSPNSQSGSSGLVTIDKQELNTQVFAKHGETIVLGGVFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYIIQSNEKLGGVKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=405171 GM698_RS01965 WP_176812332.1 440135..441448(+) (comE) [Mannheimia sp. ZY171111]
ATGTTTAGAATTTTTCTTTTTTTATTTATTAGCTCCTCATTAGCATTTGCTAACACAATTTCTCTTGCTGTGAAAAATGC
ACCCACTGCAACCATTTTGACTTATTTATCCGAAGAAATAGGAAAAAATATTGTTTTAGATGATAATATTCAAACAAAAT
CAACATTAAGATTAGAAAATAAATCCATTGACGATATATTCAAAGTAATAAGCACAATGAATAAATTATCGCTACATCAA
GAAGGTGATGTTATTTATATCAGTAAAAAAGAAGAAAAATTGACCGATTTAAAAACAATTCCAATCGCTCATTTGCAAAA
TAATATAGAAAATACACCGCTTGTTACCACACCCAAACTCATTACTAAAACAATAAAACTACATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACAAAAGGCAGCGGTACTTTTTTATCTGAAAATGGCTATATCCATTTTGATGAGCGAAGTAAT
AGTTTAATTATCAAAGACAGTACAAAATCACTTCAACACATAGAAAAGTTAGTTAAACAGCTTGACCAACCAACTGAGCA
AATTGCAATTGAAGCACGCATTGTCACAATCAGCAGTGAACATTTACAAGAATTGGGGGTGCGTTGGGGAATGTTCTCAA
AAGGGGCTGATCACTATAAATTTGGTGGGCGACTTGAAAGTAACGGATTAAGCAATGTTGCTAATAATCTCAATGTGAGT
TTTCCTGTTACGAATGGTGCTTCAGCAGTTTTACAAGTCGCTTCTATTAATAGCCGAGTACTGGATCTGGAATTGAGTGC
CTTAGAGCAAGAAAATAGTGTAGAAATTATCGCCAGCCCTCGCCTTTTGACTACGAATAAAAAACCAGCGAGTATCAAAC
AAGGGACAGAAATTCCTTATGTGATGTACAATAAAAAATCGGAAGCTACTAATGTGGAATTTAAAGAAGCCGTGCTGGGC
TTAGAAGTTATTCCTCATCTTTCGACTGAAAATCAAATATTACTCGATCTGATTGTTACTCAAAATTCTCCTAATTCGCA
ATCAGGCAGTAGTGGCTTAGTGACTATTGATAAACAAGAATTGAATACACAAGTTTTTGCGAAACACGGCGAAACGATCG
TTTTAGGCGGAGTTTTCCAACATTTAACGCAAAAAGGAGAAGATAAAGTACCTATTTTAGGTTCAATTCCCTTTATCAAA
CGCTTATTTAGCCAAACTCGAGATAAAATTAGCAAAAGAGAACTTGTGATTTTTGTTACCCCTTACATTATTCAATCAAA
TGAAAAATTAGGTGGTGTGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

61.609

99.542

0.613
  comE Haemophilus influenzae Rd KW20

49.206

100

0.497
  comE Haemophilus influenzae 86-028NP

48.98

100

0.494
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.623

97.025

0.384
  pilQ Vibrio cholerae strain A1552

39.623

97.025

0.384
  pilQ Vibrio campbellii strain DS40M4

38.462

98.169

0.378

Multiple sequence alignment