Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   GM695_RS05050 Genome accession   NZ_CP046531
Coordinates   996328..997641 (-) Length   437 a.a.
NCBI ID   WP_159629284.1    Uniprot ID   A0A7D5HTS3
Organism   Mannheimia pernigra strain ZY170218     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 991328..1002641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM695_RS05020 (GM695_05015) ruvB 991957..992961 (-) 1005 WP_159629278.1 Holliday junction branch migration DNA helicase RuvB -
  GM695_RS05025 (GM695_05020) ruvA 992972..993574 (-) 603 WP_159629279.1 Holliday junction branch migration protein RuvA -
  GM695_RS05030 (GM695_05025) - 993683..994309 (-) 627 WP_159629280.1 LysE family transporter -
  GM695_RS05035 (GM695_05030) - 994303..994785 (-) 483 WP_159629281.1 phosphatidylglycerophosphatase A -
  GM695_RS05040 (GM695_05035) thiL 994791..995771 (-) 981 WP_159629282.1 thiamine-phosphate kinase -
  GM695_RS05045 (GM695_05040) nusB 995835..996248 (-) 414 WP_159629283.1 transcription antitermination factor NusB -
  GM695_RS05050 (GM695_05045) comE 996328..997641 (-) 1314 WP_159629284.1 type IV pilus secretin PilQ Machinery gene
  GM695_RS05055 (GM695_05050) - 997650..998045 (-) 396 WP_159629285.1 hypothetical protein -
  GM695_RS05060 (GM695_05055) - 998047..998580 (-) 534 WP_159629286.1 hypothetical protein -
  GM695_RS05065 (GM695_05060) - 998582..999103 (-) 522 WP_159629287.1 hypothetical protein -
  GM695_RS05070 (GM695_05065) - 999079..999789 (-) 711 WP_159629288.1 competence protein ComA -
  GM695_RS05075 (GM695_05070) - 999916..1002465 (+) 2550 WP_159629289.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48415.60 Da        Isoelectric Point: 8.9535

>NTDB_id=405155 GM695_RS05050 WP_159629284.1 996328..997641(-) (comE) [Mannheimia pernigra strain ZY170218]
MFRIFLFLFISSSLAFANTISLAVKNAPTATILTYLSEEIGKNIVLDDNIQTKSTLRLENKSIDDIFKVISTMNKLSLHQ
EGDVIYISKKEEKLTDLKTIPIAHLQNNIENTPLVTTPKLITKTIKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSTKSLQHIEKLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSKGADHYKFGGRLESNGLSNVANNLNVS
FPVTNGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNKKSEATNVEFKEAVLG
LEVTPHLSTENQILLDLIVTQNSPNSQSGSSGLVTIDKQELNTQVFAKHGETIVLGGVFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYIIQSNEKLGGVKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=405155 GM695_RS05050 WP_159629284.1 996328..997641(-) (comE) [Mannheimia pernigra strain ZY170218]
ATGTTTAGAATTTTTCTTTTTTTATTTATTAGCTCCTCATTAGCATTTGCTAACACAATTTCTCTTGCTGTGAAAAATGC
CCCCACTGCAACCATTTTGACTTATTTATCCGAAGAAATAGGAAAAAATATTGTTTTAGATGATAATATTCAAACAAAAT
CAACATTAAGATTAGAAAATAAATCCATTGACGATATATTCAAAGTAATAAGCACAATGAATAAATTATCGCTACATCAA
GAAGGTGATGTTATTTATATCAGTAAAAAAGAAGAAAAATTGACCGATTTAAAAACAATTCCAATCGCTCATTTGCAAAA
TAATATAGAAAATACACCGCTTGTTACCACACCCAAACTCATTACTAAAACAATAAAACTACATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACAAAAGGCAGCGGTACTTTTTTATCTGAAAATGGCTATATCCATTTTGATGAGCGAAGTAAT
AGTTTAATTATCAAAGACAGTACAAAATCACTTCAACACATAGAAAAGTTAGTTAAACAGCTTGACCAACCAACTGAGCA
AATTGCAATTGAAGCACGCATTGTCACAATCAGCAGTGAACATTTACAAGAATTGGGGGTGCGTTGGGGAATGTTCTCAA
AAGGGGCTGATCACTATAAATTTGGTGGGCGACTTGAAAGTAACGGATTAAGCAATGTTGCTAATAATCTCAATGTGAGT
TTTCCTGTTACGAATGGTGCTTCAGCAGTTTTACAAGTCGCTTCTATTAATAGCCGAGTACTGGATCTGGAATTGAGTGC
CTTAGAGCAAGAAAATAGTGTAGAAATTATTGCCAGCCCTCGCCTTTTGACTACGAATAAAAAACCAGCGAGTATCAAAC
AAGGGACAGAAATTCCTTATGTGATGTACAATAAAAAATCGGAAGCTACTAATGTGGAATTTAAAGAAGCCGTGCTGGGC
TTAGAAGTTACTCCTCATCTTTCGACTGAAAATCAAATATTACTCGATCTGATTGTTACTCAAAATTCTCCTAATTCGCA
ATCAGGCAGTAGTGGCTTAGTGACTATTGATAAACAAGAATTGAATACACAAGTTTTTGCGAAACACGGCGAAACAATCG
TTTTAGGCGGAGTCTTCCAACATTTAACGCAAAAAGGAGAAGATAAAGTACCTATTTTAGGTTCAATTCCGTTTATCAAA
CGCTTATTCAGCCAAACTCGAGATAAAATTAGCAAAAGAGAACTTGTGATTTTTGTTACCCCTTACATTATTCAATCAAA
TGAAAAATTAGGTGGTGTGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5HTS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

61.839

99.542

0.616
  comE Haemophilus influenzae Rd KW20

49.433

100

0.499
  comE Haemophilus influenzae 86-028NP

49.206

100

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.858

97.025

0.387
  pilQ Vibrio cholerae strain A1552

39.858

97.025

0.387
  pilQ Vibrio campbellii strain DS40M4

38.695

98.169

0.38

Multiple sequence alignment