Detailed information NTG0405153

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HWQ57_RS24355
• Genome accession: NZ_CP058333
• Coordinates: 5211489..5212637 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ACR20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5206489..5217637

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWQ57_RS24335 (HWQ57_24135)	-	5207227..5208411 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
HWQ57_RS24340 (HWQ57_24140)	-	5208408..5209781 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HWQ57_RS24345 (HWQ57_24145)	-	5210024..5210428 (-)	405	WP_003084560.1	NINE protein	-
HWQ57_RS24350 (HWQ57_24150)	czcD	5210582..5211481 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HWQ57_RS24355 (HWQ57_24155)	pilU	5211489..5212637 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HWQ57_RS24360 (HWQ57_24160)	pilT	5212815..5213849 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HWQ57_RS24365 (HWQ57_24165)	-	5214064..5214756 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HWQ57_RS24370 (HWQ57_24170)	proC	5214768..5215589 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HWQ57_RS24375 (HWQ57_24175)	-	5215600..5216193 (+)	594	WP_003084544.1	YggT family protein	-
HWQ57_RS24380	-	5216190..5216486 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=405153 HWQ57_RS24355 WP_003100959.1 5211489..5212637(-) (pilU) [Pseudomonas aeruginosa strain ACR20]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=405153 HWQ57_RS24355 WP_003100959.1 5211489..5212637(-) (pilU) [Pseudomonas aeruginosa strain ACR20]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382