Detailed information NTG0404910

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HWQ50_RS04630
• Genome accession: NZ_CP058331
• Coordinates: 996772..997920 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ACR22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 991772..1002920

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWQ50_RS04605	-	992923..993219 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HWQ50_RS04610 (HWQ50_04605)	-	993216..993809 (-)	594	WP_003084544.1	YggT family protein	-
HWQ50_RS04615 (HWQ50_04610)	proC	993820..994641 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HWQ50_RS04620 (HWQ50_04615)	-	994653..995345 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HWQ50_RS04625 (HWQ50_04620)	pilT	995560..996594 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HWQ50_RS04630 (HWQ50_04625)	pilU	996772..997920 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HWQ50_RS04635 (HWQ50_04630)	czcD	997928..998827 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HWQ50_RS04640 (HWQ50_04635)	-	998981..999385 (+)	405	WP_003084560.1	NINE protein	-
HWQ50_RS04645 (HWQ50_04640)	-	999628..1001001 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HWQ50_RS04650 (HWQ50_04645)	-	1000998..1002182 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=404910 HWQ50_RS04630 WP_003100959.1 996772..997920(+) (pilU) [Pseudomonas aeruginosa strain ACR22]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=404910 HWQ50_RS04630 WP_003100959.1 996772..997920(+) (pilU) [Pseudomonas aeruginosa strain ACR22]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382