Detailed information NTG0404460

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HTZ92_RS13180
• Genome accession: NZ_CP058289
• Coordinates: 2783410..2784528 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 strain ATCC 19606
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2778410..2789528

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HTZ92_RS13170 (HTZ92_2585)	-	2781526..2782218 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
HTZ92_RS13175 (HTZ92_2586)	pilT	2782345..2783382 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HTZ92_RS13180 (HTZ92_2587)	pilU	2783410..2784528 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HTZ92_RS13185 (HTZ92_2588)	fur	2784598..2785035 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HTZ92_RS13190 (HTZ92_2589)	-	2785147..2785545 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HTZ92_RS13195 (HTZ92_2590)	-	2785600..2785923 (-)	324	WP_000442584.1	RnfH family protein	-
HTZ92_RS13200 (HTZ92_2591)	-	2785923..2786990 (-)	1068	WP_005144332.1	hypothetical protein	-
HTZ92_RS13205 (HTZ92_2592)	-	2787143..2787598 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HTZ92_RS13210 (HTZ92_2593)	-	2787851..2788699 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=404460 HTZ92_RS13180 WP_000347036.1 2783410..2784528(+) (pilU) [Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 strain ATCC 19606]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=404460 HTZ92_RS13180 WP_000347036.1 2783410..2784528(+) (pilU) [Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 strain ATCC 19606]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392