Detailed information NTG0040414

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MDS_RS22755
• Genome accession: NC_015410
• Coordinates: 4909681..4910826 (-)
• Length: 381 a.a.
• NCBI ID: WP_013717518.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina NK-01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4904681..4915826

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MDS_RS22735 (MDS_4470)	-	4906063..4906707 (-)	645	WP_013717514.1	DUF2076 domain-containing protein	-
MDS_RS22740 (MDS_4471)	-	4906817..4907215 (-)	399	WP_013717515.1	TM2 domain-containing protein	-
MDS_RS22745 (MDS_4472)	-	4907363..4908574 (+)	1212	WP_013717516.1	putative DNA modification/repair radical SAM protein	-
MDS_RS22750 (MDS_4473)	-	4908574..4909434 (+)	861	WP_013717517.1	TIGR03915 family putative DNA repair protein	-
MDS_RS22755 (MDS_4474)	pilU	4909681..4910826 (-)	1146	WP_013717518.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MDS_RS22760 (MDS_4475)	pilT	4910913..4911947 (-)	1035	WP_013717519.1	type IV pilus twitching motility protein PilT	Machinery gene
MDS_RS22765 (MDS_4476)	-	4912023..4912715 (+)	693	WP_013717520.1	YggS family pyridoxal phosphate-dependent enzyme	-
MDS_RS22770 (MDS_4477)	proC	4912733..4913551 (+)	819	WP_013717521.1	pyrroline-5-carboxylate reductase	-
MDS_RS22775 (MDS_4478)	-	4913563..4914156 (+)	594	WP_013717522.1	YggT family protein	-
MDS_RS22780 (MDS_4479)	-	4914159..4914449 (+)	291	WP_013717523.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42469.79 Da
• Isoelectric Point: 6.5931

>NTDB_id=40414 MDS_RS22755 WP_013717518.1 4909681..4910826(-) (pilU) [Ectopseudomonas mendocina NK-01]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEMTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIDELKLPDILKKLSLTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSNEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=40414 MDS_RS22755 WP_013717518.1 4909681..4910826(-) (pilU) [Ectopseudomonas mendocina NK-01]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGTTCCGACCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCGCCGGAGATGACCCGCGAAACCGTGCTGGGTG
TGATGAACGAGGCGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGTCGC
TTCCGTGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGTATCGAGACCACCATCCCGACCATCGA
TGAGCTGAAGCTGCCGGACATCCTCAAGAAACTGTCATTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCGATGATCGGCTACCGTAACCGCAATAGCAGCGGCCACATCATCTCCATCGAAGAC
CCTATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACGCAGCGCGAAGTGGGTATCGACACCGAGTCGTTCGAGGT
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTACGGACCCGCGAGACCATGGATCACG
CCGTGGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGTTGGACCGCATC
ATCAACTTCTTCCCAGCCGACCGGCAGAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGACGTCGCGCGGTGATCGAGGTGCTGATCAATACCCCGCTGGCTGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCAACGAGCAGGGCATGCAGACCTTCGACCAGGCC
TTGTACAATCTCTATACCCAGGGTGAGATCACCTACGAAGATGCGCTGCTCTACGCCGATTCGGCCAACGATCTGCGTCT
GATGATCAAGCTCGGCTCTGAAACCGATGGCGATCACCTGACCAGCATGGCCCAGGGCCTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGGCGCTTTCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	63.585	93.701	0.596
pilU	Vibrio cholerae strain A1552	58.286	91.864	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383