Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HWH78_RS29575 Genome accession   NZ_CP056774
Coordinates   6356600..6357223 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain CDN129     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6351600..6362223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWH78_RS29560 (HWH78_29560) - 6352290..6354758 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  HWH78_RS29565 (HWH78_29565) pilM 6354943..6356007 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HWH78_RS29570 (HWH78_29570) pilN 6356007..6356603 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HWH78_RS29575 (HWH78_29575) pilO 6356600..6357223 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HWH78_RS29580 (HWH78_29580) comL/pilP 6357220..6357744 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HWH78_RS29585 (HWH78_29585) pilQ 6357798..6359942 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  HWH78_RS29590 (HWH78_29590) aroK 6359954..6360472 (+) 519 WP_003095833.1 shikimate kinase AroK -
  HWH78_RS29595 (HWH78_29595) aroB 6360522..6361628 (+) 1107 WP_003162482.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=403564 HWH78_RS29575 WP_003103268.1 6356600..6357223(+) (pilO) [Pseudomonas aeruginosa strain CDN129]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=403564 HWH78_RS29575 WP_003103268.1 6356600..6357223(+) (pilO) [Pseudomonas aeruginosa strain CDN129]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401