Detailed information NTG0040356

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: SPYALAB49_RS00335
• Genome accession: NC_017596
• Coordinates: 54529..55527 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes Alab49
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49529..60527

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYALAB49_RS00320 (SPYALAB49_000061)	-	50265..51605 (+)	1341	WP_014635219.1	hypothetical protein	-
SPYALAB49_RS00325 (SPYALAB49_000063)	purB	51975..53267 (+)	1293	WP_002986683.1	adenylosuccinate lyase	-
SPYALAB49_RS00330 (SPYALAB49_000064)	comR	53398..54309 (+)	912	WP_014635220.1	XRE family transcriptional regulator	Regulator
SPYALAB49_RS09870	comS	54400..54498 (+)	99	WP_020833188.1	quorum-sensing system DWW-type pheromone	Regulator
SPYALAB49_RS00335 (SPYALAB49_000065)	ruvB	54529..55527 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
SPYALAB49_RS00340 (SPYALAB49_000066)	-	55665..56102 (+)	438	WP_014635221.1	low molecular weight protein-tyrosine-phosphatase	-
SPYALAB49_RS00345 (SPYALAB49_000067)	-	56125..56526 (+)	402	WP_012560369.1	MORN repeat-containing protein	-
SPYALAB49_RS00350 (SPYALAB49_000068)	-	56523..58298 (+)	1776	WP_014635222.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=40356 SPYALAB49_RS00335 WP_002986679.1 54529..55527(+) (ruvB) [Streptococcus pyogenes Alab49]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=40356 SPYALAB49_RS00335 WP_002986679.1 54529..55527(+) (ruvB) [Streptococcus pyogenes Alab49]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTGCTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473