Detailed information NTG0403440

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: GL189_RS09655
• Genome accession: NZ_CP046360
• Coordinates: 1880657..1881118 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250539.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain 574
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1875657..1886118

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GL189_RS09625 (GL189_09790)	-	1875970..1876794 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
GL189_RS09630 (GL189_09795)	rnpA	1876769..1877140 (-)	372	WP_000739247.1	ribonuclease P protein component	-
GL189_RS11185	-	1877157..1877288 (-)	132	WP_000768904.1	hypothetical protein	-
GL189_RS09635 (GL189_09800)	-	1877289..1878479 (-)	1191	WP_000167765.1	acetate kinase	-
GL189_RS09640 (GL189_09805)	comYH	1878530..1879483 (-)	954	WP_000345122.1	class I SAM-dependent methyltransferase	Machinery gene
GL189_RS09645 (GL189_09810)	-	1879544..1880131 (-)	588	WP_000679779.1	class I SAM-dependent methyltransferase	-
GL189_RS09650 (GL189_09815)	comGG/cglG	1880287..1880679 (-)	393	WP_001818028.1	competence type IV pilus minor pilin ComGG	Machinery gene
GL189_RS09655 (GL189_09820)	comGF/cglF	1880657..1881118 (-)	462	WP_000250539.1	competence type IV pilus minor pilin ComGF	Machinery gene
GL189_RS09660 (GL189_09825)	comGE/cglE	1881081..1881383 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
GL189_RS09665 (GL189_09830)	comGD/cglD	1881346..1881750 (-)	405	WP_000588030.1	competence type IV pilus minor pilin ComGD	Machinery gene
GL189_RS09670 (GL189_09835)	comGC/cglC	1881743..1882069 (-)	327	WP_000738625.1	competence type IV pilus major pilin ComGC	Machinery gene
GL189_RS09675 (GL189_09840)	comGB/cglB	1882071..1883087 (-)	1017	WP_074017570.1	competence type IV pilus assembly protein ComGB	Machinery gene
GL189_RS09680 (GL189_09845)	comGA/cglA/cilD	1883035..1883976 (-)	942	WP_000249555.1	competence type IV pilus ATPase ComGA	Machinery gene
GL189_RS09685 (GL189_09850)	-	1884051..1884416 (-)	366	WP_000286415.1	DUF1033 family protein	-
GL189_RS09690 (GL189_09855)	-	1884567..1885625 (-)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17851.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=403440 GL189_RS09655 WP_000250539.1 1880657..1881118(-) (comGF/cglF) [Streptococcus pneumoniae strain 574]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=403440 GL189_RS09655 WP_000250539.1 1880657..1881118(-) (comGF/cglF) [Streptococcus pneumoniae strain 574]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae D39	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae R6	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae TIGR4	94.118	100	0.941
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915