Detailed information NTG0040309

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BGLA_RS17425
• Genome accession: NC_015381
• Coordinates: 3980557..3981735 (+)
• Length: 392 a.a.
• NCBI ID: WP_013699425.1
• Uniprot ID: F2LD21
• Organism: Burkholderia gladioli BSR3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3975557..3986735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BGLA_RS17395 (bgla_1g34990)	-	3976175..3976789 (+)	615	WP_013699419.1	50S ribosomal protein L25/general stress protein Ctc	-
BGLA_RS17400 (bgla_1g35000)	pth	3976886..3977491 (+)	606	WP_013699420.1	aminoacyl-tRNA hydrolase	-
BGLA_RS17405 (bgla_1g35010)	hisC	3977587..3978654 (+)	1068	WP_013699421.1	histidinol-phosphate transaminase	-
BGLA_RS17410 (bgla_1g35020)	-	3978801..3979073 (-)	273	WP_013699422.1	YfhL family 4Fe-4S dicluster ferredoxin	-
BGLA_RS17415 (bgla_1g35030)	coaD	3979140..3979640 (-)	501	WP_013699423.1	pantetheine-phosphate adenylyltransferase	-
BGLA_RS17420 (bgla_1g35040)	rsmD	3979752..3980366 (-)	615	WP_013699424.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BGLA_RS17425 (bgla_1g35050)	pilA	3980557..3981735 (+)	1179	WP_013699425.1	signal recognition particle-docking protein FtsY	Machinery gene
BGLA_RS17430 (bgla_1g35060)	maiA	3981808..3982452 (-)	645	WP_013699426.1	maleylacetoacetate isomerase	-
BGLA_RS17435 (bgla_1g35070)	ybiB	3982666..3983646 (+)	981	WP_013699427.1	DNA-binding protein YbiB	-
BGLA_RS17440 (bgla_1g35080)	leuA	3984146..3985798 (+)	1653	WP_013699428.1	2-isopropylmalate synthase	-
BGLA_RS17445 (bgla_1g35090)	-	3985981..3986367 (-)	387	WP_013699429.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 41249.26 Da
• Isoelectric Point: 4.7373

>NTDB_id=40309 BGLA_RS17425 WP_013699425.1 3980557..3981735(+) (pilA) [Burkholderia gladioli BSR3]
MFSFFKRLIGSKADGAPTDDPDAPTDRQDDAEAPAAEAAWPVERVAAPAPAVPVEPAAPAQVAPALPAATANDDEAQAVT
AVEIVPPPAPEPAAKKSWIARLRSGLSKTSSSLTSVFVSTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVKAERL
TDPQQVKAALRGLLIALLAPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLA
IWGERNNVTVVQQESGDPAAVIFDAVGAARARKINVVMADTAGRLPTQLHLMEELRKVKRVIGKAQADAPHEVLLVIDAN
TGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAEEFTDALLG

Nucleotide

• Length: 1179 bp

>NTDB_id=40309 BGLA_RS17425 WP_013699425.1 3980557..3981735(+) (pilA) [Burkholderia gladioli BSR3]
ATGTTCAGCTTCTTCAAACGATTGATCGGCTCGAAGGCCGACGGCGCGCCGACCGACGACCCGGACGCGCCGACGGACCG
GCAGGACGACGCCGAGGCCCCCGCGGCCGAAGCGGCCTGGCCCGTCGAGCGGGTCGCCGCGCCTGCCCCGGCGGTTCCGG
TCGAGCCGGCCGCGCCCGCGCAAGTCGCCCCAGCTCTCCCCGCCGCCACCGCGAACGACGACGAAGCGCAGGCCGTCACG
GCCGTCGAGATCGTGCCGCCGCCCGCGCCGGAGCCGGCCGCCAAGAAATCCTGGATCGCTCGCCTGCGCTCGGGCCTGTC
GAAGACCAGTTCCAGCCTGACCAGCGTCTTCGTCAGCACCAAGATCGACGAGGATCTCTACGAGGAGCTCGAGACCGCGC
TGCTGATGTCCGACGCCGGCGTCGACGCCACCGAGTACCTGCTCGGCGCGCTGCGCGAGAAGGTCAAGGCCGAGCGGCTG
ACCGATCCGCAGCAGGTCAAGGCGGCCCTGCGCGGCCTGCTGATCGCGCTGCTCGCGCCGCTCGAGAAGTCACTGATGCT
GGGTCGCGCGCAGCCGCTGGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTGGCCA
AGCATCTGCAGAGCTTCGACCAGTCGGTGCTGCTGGCCGCCGGGGACACCTTCCGCGCCGCCGCGCGCGAGCAGCTCGCG
ATCTGGGGCGAGCGCAACAACGTGACGGTGGTGCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTGGG
CGCGGCGCGCGCGCGCAAGATCAACGTGGTGATGGCCGATACGGCGGGCCGCCTGCCCACCCAGCTGCACCTGATGGAGG
AGCTGCGCAAGGTCAAGCGCGTGATCGGCAAGGCACAGGCCGACGCGCCGCACGAGGTGCTGCTGGTGATCGACGCCAAC
ACCGGCCAGAACGCGCTCGCCCAGGTAAAAGCCTTCGACGACGCGCTGGGCCTCACCGGCCTGATCGTCACCAAGCTCGA
CGGCACCGCGAAGGGCGGCATCCTCGCCGCGATCGCGCGGCAGCGGCCGGTGCCGGTCTACTTCATCGGCGTCGGCGAGA
AGGTGGAGGACCTGCAGCCCTTCAATGCCGAGGAGTTCACCGACGCGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (193-391)

• SRP54-type protein, helical bundle domain (SRP54_N) (111-171)

3D structure

• AlphaFold DB: F2LD21

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	56.284	93.367	0.526