Detailed information NTG0402687

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HUZ70_RS19430
• Genome accession: NZ_CP055048
• Coordinates: 4068380..4069360 (+)
• Length: 326 a.a.
• NCBI ID: WP_001598608.1
• Uniprot ID: -
• Organism: Escherichia coli strain SWHEFF_58
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4063380..4074360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUZ70_RS19400 (HUZ70_19235)	-	4063757..4064743 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
HUZ70_RS19405 (HUZ70_19240)	hemW	4065057..4066193 (-)	1137	WP_001521257.1	radical SAM family heme chaperone HemW	-
HUZ70_RS19410 (HUZ70_19245)	rdgB	4066186..4066779 (-)	594	WP_001174747.1	XTP/dITP diphosphatase	-
HUZ70_RS19415 (HUZ70_19250)	yggU	4066787..4067077 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
HUZ70_RS19420 (HUZ70_19255)	yggT	4067074..4067640 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
HUZ70_RS19425 (HUZ70_19260)	yggS	4067658..4068362 (-)	705	WP_001598609.1	pyridoxal phosphate homeostasis protein	-
HUZ70_RS19430 (HUZ70_19265)	pilT	4068380..4069360 (+)	981	WP_001598608.1	type IV pilus twitching motility protein PilT	Machinery gene
HUZ70_RS19435 (HUZ70_19270)	ruvX	4069475..4069891 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
HUZ70_RS19440 (HUZ70_19275)	yqgE	4069891..4070454 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
HUZ70_RS19445 (HUZ70_19280)	gshB	4070563..4071513 (-)	951	WP_000593273.1	glutathione synthase	-
HUZ70_RS19450 (HUZ70_19285)	rsmE	4071526..4072257 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
HUZ70_RS19455 (HUZ70_19290)	endA	4072337..4073044 (-)	708	WP_000286497.1	deoxyribonuclease I	-
HUZ70_RS19460 (HUZ70_19295)	yggI	4073139..4073636 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35928.10 Da
• Isoelectric Point: 5.7980

>NTDB_id=402687 HUZ70_RS19430 WP_001598608.1 4068380..4069360(+) (pilT) [Escherichia coli strain SWHEFF_58]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=402687 HUZ70_RS19430 WP_001598608.1 4068380..4069360(+) (pilT) [Escherichia coli strain SWHEFF_58]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGAGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGTTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362