Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HUZ70_RS18845 Genome accession   NZ_CP055048
Coordinates   3948285..3949778 (+) Length   497 a.a.
NCBI ID   WP_000249326.1    Uniprot ID   -
Organism   Escherichia coli strain SWHEFF_58     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3943285..3954778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUZ70_RS18825 (HUZ70_18675) pilD 3943933..3944742 (+) 810 WP_061090010.1 prepilin peptidase PppA Machinery gene
  HUZ70_RS18830 (HUZ70_18680) gspS2 3944808..3945218 (+) 411 WP_061089992.1 type II secretion system pilot lipoprotein GspS-beta -
  HUZ70_RS18835 (HUZ70_18685) gspC 3945236..3946195 (+) 960 WP_024211239.1 type II secretion system protein GspC -
  HUZ70_RS18840 (HUZ70_18690) gspD 3946225..3948285 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  HUZ70_RS18845 (HUZ70_18695) pilB/pilB1 3948285..3949778 (+) 1494 WP_000249326.1 type II secretion system ATPase GspE Machinery gene
  HUZ70_RS18850 (HUZ70_18700) gspF 3949778..3951001 (+) 1224 WP_061089993.1 type II secretion system inner membrane protein GspF -
  HUZ70_RS18855 (HUZ70_18705) gspG 3951018..3951473 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  HUZ70_RS18860 (HUZ70_18710) gspH 3951477..3952040 (+) 564 WP_001115129.1 type II secretion system minor pseudopilin GspH -
  HUZ70_RS18865 (HUZ70_18715) gspI 3952037..3952408 (+) 372 WP_000820101.1 type II secretion system minor pseudopilin GspI -
  HUZ70_RS18870 (HUZ70_18720) gspJ 3952405..3953010 (+) 606 WP_001250454.1 type II secretion system minor pseudopilin GspJ -
  HUZ70_RS18875 (HUZ70_18725) gspK 3953007..3953984 (+) 978 WP_000633196.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55257.42 Da        Isoelectric Point: 6.0089

>NTDB_id=402683 HUZ70_RS18845 WP_000249326.1 3948285..3949778(+) (pilB/pilB1) [Escherichia coli strain SWHEFF_58]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLNHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQALPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=402683 HUZ70_RS18845 WP_000249326.1 3948285..3949778(+) (pilB/pilB1) [Escherichia coli strain SWHEFF_58]
ATGGTGCCTGTAGCACAGGAAACCACTGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCATCGTTGGAGATCCTCCACGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGTGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGACGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAACCATGAACTGACC
GCGCAGTTACGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGTACGCTGTGCCCCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTCCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Legionella pneumophila strain ERS1305867

43.852

86.72

0.38
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment