Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GLX29_RS20480 Genome accession   NZ_CP046251
Coordinates   4336445..4337878 (+) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain P12375     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4331445..4342878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLX29_RS20450 (GLX29_20450) - 4331991..4332968 (+) 978 WP_032614470.1 calcium/sodium antiporter -
  GLX29_RS20455 (GLX29_20455) kdsD 4332982..4333968 (+) 987 WP_155167427.1 arabinose-5-phosphate isomerase KdsD -
  GLX29_RS20460 (GLX29_20460) kdsC 4333983..4334549 (+) 567 WP_032614474.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GLX29_RS20465 (GLX29_20465) lptC 4334546..4335121 (+) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  GLX29_RS20470 (GLX29_20470) lptA 4335090..4335665 (+) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  GLX29_RS20475 (GLX29_20475) lptB 4335672..4336397 (+) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  GLX29_RS20480 (GLX29_20480) rpoN/rpoN1 4336445..4337878 (+) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  GLX29_RS20485 (GLX29_20485) hpf 4337901..4338188 (+) 288 WP_155167429.1 ribosome hibernation promoting factor -
  GLX29_RS20490 (GLX29_20490) ptsN 4338271..4338762 (+) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GLX29_RS20495 (GLX29_20495) rapZ 4338808..4339662 (+) 855 WP_032614487.1 RNase adapter RapZ -
  GLX29_RS20500 (GLX29_20500) npr 4339659..4339931 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  GLX29_RS20505 (GLX29_20505) mtgA 4339928..4340653 (-) 726 WP_155167431.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GLX29_RS20510 (GLX29_20510) elbB 4340650..4341303 (-) 654 WP_151586562.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=401996 GLX29_RS20480 WP_032614481.1 4336445..4337878(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain P12375]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=401996 GLX29_RS20480 WP_032614481.1 4336445..4337878(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain P12375]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGTACGCCATCCGGTACGCGCGCCGATTACCAGGATGA
TGAGTTACCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACACCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGCGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACAAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAATTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTAGAGCAACAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCTTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment