Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GJQ55_RS10725 Genome accession   NZ_CP046056
Coordinates   2265046..2265924 (+) Length   292 a.a.
NCBI ID   WP_228344963.1    Uniprot ID   A0A9X7UWG3
Organism   Venatoribacter cucullus strain ASxL5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2260046..2270924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJQ55_RS10710 (GJQ55_10635) - 2260153..2261997 (+) 1845 WP_228344960.1 sulfatase-like hydrolase/transferase -
  GJQ55_RS10715 (GJQ55_10640) pilB 2262074..2263774 (+) 1701 WP_228344961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GJQ55_RS10720 (GJQ55_10645) pilC 2263820..2265046 (+) 1227 WP_228344962.1 type II secretion system F family protein Machinery gene
  GJQ55_RS10725 (GJQ55_10650) pilD 2265046..2265924 (+) 879 WP_228344963.1 prepilin peptidase Machinery gene
  GJQ55_RS10730 (GJQ55_10655) coaE 2265924..2266523 (+) 600 WP_420907144.1 dephospho-CoA kinase -
  GJQ55_RS10735 (GJQ55_10660) yacG 2266644..2266844 (+) 201 WP_228344964.1 DNA gyrase inhibitor YacG -
  GJQ55_RS10740 (GJQ55_10665) - 2266960..2267418 (-) 459 WP_228344965.1 copper chaperone PCu(A)C -
  GJQ55_RS10745 (GJQ55_10670) - 2267550..2269511 (-) 1962 WP_228344966.1 EAL domain-containing protein -
  GJQ55_RS10750 (GJQ55_10675) - 2269562..2270230 (-) 669 WP_228344967.1 transglutaminase-like cysteine peptidase -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32028.18 Da        Isoelectric Point: 8.0462

>NTDB_id=400742 GJQ55_RS10725 WP_228344963.1 2265046..2265924(+) (pilD) [Venatoribacter cucullus strain ASxL5]
MSTLAFIGNEPWLAVLSLVVLGLLIGSFLNVVILRFPIMLFRGWKRDCEEMVSTLPDHPALANMDKPFNLLKPDSHCPKC
HAPVRAWQNIPVISWLLLRGKCASCGTRIGWRYPAVELATALFSAVLLLVFPWGWQLAAMLVFTWLLISMAVIDIDHQIL
PDTMTLGLMWLGLAINTQGLFTDLTSAVWGAIGGYGLLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGVQSLPLIILL
SSVVGAVVGIAGILLQGRDKNVPIPFGPYLAAAGWIAALWGNDITRWYVGHF

Nucleotide


Download         Length: 879 bp        

>NTDB_id=400742 GJQ55_RS10725 WP_228344963.1 2265046..2265924(+) (pilD) [Venatoribacter cucullus strain ASxL5]
ATGTCCACCCTCGCATTTATCGGCAACGAACCCTGGCTGGCCGTACTCAGCCTGGTGGTACTGGGCCTGCTGATCGGCAG
CTTTCTGAATGTGGTGATTCTGCGCTTTCCCATCATGCTGTTCCGGGGCTGGAAGCGCGATTGTGAAGAGATGGTCAGCA
CCTTGCCCGACCATCCGGCGCTGGCCAACATGGACAAGCCGTTTAACCTGCTGAAACCCGATTCCCATTGCCCGAAATGC
CACGCGCCGGTACGAGCCTGGCAGAATATTCCGGTCATCAGCTGGTTGCTGTTGCGCGGTAAATGTGCCAGCTGCGGCAC
CCGCATTGGCTGGCGTTACCCGGCGGTCGAACTGGCCACCGCGCTGTTCAGTGCGGTATTGCTGCTGGTCTTTCCCTGGG
GCTGGCAACTGGCCGCCATGCTGGTATTTACCTGGCTGTTGATCAGCATGGCCGTGATTGATATCGACCACCAGATTCTG
CCCGACACCATGACGCTGGGCCTGATGTGGCTGGGGCTGGCCATTAACACGCAGGGCCTGTTTACCGATTTAACCAGCGC
CGTCTGGGGTGCCATTGGGGGTTACGGTTTACTGTGGTCGGTGTTCTGGTTATTCAAACTGGCCACCGGCAAAGAAGGCA
TGGGCTACGGTGACTTTAAACTGCTGGCCGCGCTGGGCGCCTGGCTGGGTGTACAAAGCCTGCCCCTGATTATTCTGCTG
TCGTCCGTCGTGGGTGCGGTGGTGGGAATTGCCGGCATTCTGCTGCAGGGCCGCGATAAAAACGTCCCCATTCCCTTTGG
TCCGTATCTGGCCGCCGCCGGCTGGATTGCGGCACTGTGGGGCAACGACATTACCCGCTGGTACGTCGGACACTTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(141-250)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

51.579

97.603

0.503
  pilD Acinetobacter baumannii D1279779

51.579

97.603

0.503
  pilD Vibrio campbellii strain DS40M4

49.462

95.548

0.473
  pilD Vibrio cholerae strain A1552

48.746

95.548

0.466
  pilD Neisseria gonorrhoeae MS11

49.091

94.178

0.462

Multiple sequence alignment