Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   GJS32_RS00710 Genome accession   NZ_CP046040
Coordinates   122550..122984 (+) Length   144 a.a.
NCBI ID   WP_014622144.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain OH-71905     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 117550..127984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJS32_RS00680 (GJS32_00680) - 118859..119527 (+) 669 WP_014622139.1 ATP-binding cassette domain-containing protein -
  GJS32_RS00685 (GJS32_00685) comGA/cglA 119684..120622 (+) 939 WP_038674626.1 competence type IV pilus ATPase ComGA Machinery gene
  GJS32_RS00690 (GJS32_00690) comYB 120555..121589 (+) 1035 WP_228269618.1 competence type IV pilus assembly protein ComGB Machinery gene
  GJS32_RS00695 (GJS32_00695) comGC/cglC 121590..121913 (+) 324 WP_014622142.1 competence type IV pilus major pilin ComGC Machinery gene
  GJS32_RS00700 (GJS32_00700) comGD 121891..122316 (+) 426 WP_012678812.1 competence type IV pilus minor pilin ComGD -
  GJS32_RS00705 (GJS32_00705) comGE 122321..122563 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  GJS32_RS00710 (GJS32_00710) comYF 122550..122984 (+) 435 WP_014622144.1 competence type IV pilus minor pilin ComGF Machinery gene
  GJS32_RS10505 comGG 122962..123141 (+) 180 WP_014622145.1 competence type IV pilus minor pilin ComGG -
  GJS32_RS00720 (GJS32_00720) comYH 123386..124339 (+) 954 WP_014622146.1 class I SAM-dependent methyltransferase Machinery gene
  GJS32_RS00725 (GJS32_00725) - 124399..125598 (+) 1200 WP_012678817.1 acetate kinase -
  GJS32_RS00730 (GJS32_00730) - 125963..126574 (-) 612 WP_014622147.1 hypothetical protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16185.72 Da        Isoelectric Point: 10.1988

>NTDB_id=400443 GJS32_RS00710 WP_014622144.1 122550..122984(+) (comYF) [Streptococcus equi subsp. zooepidemicus strain OH-71905]
MKDSRLKAFTLIECLIALLVISGSLLVYQALTKSLMVSERYLAANDQDNWLLFSQQLRAELSGTTLQGVSNNRLYVEKDK
KTLSFGQVKSHDFRKAAGNGRGYQPMLFGLSSSQITTVGQQVIIKLKWQSGLERTFIYAFQEKG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=400443 GJS32_RS00710 WP_014622144.1 122550..122984(+) (comYF) [Streptococcus equi subsp. zooepidemicus strain OH-71905]
TTGAAAGACAGTAGGCTAAAGGCTTTCACCTTGATAGAGTGCCTTATTGCCTTGCTTGTCATCTCAGGCTCTTTATTAGT
TTATCAGGCCTTAACCAAGAGCCTTATGGTGAGTGAGAGGTATCTAGCAGCAAATGATCAGGACAACTGGCTTTTATTTT
CCCAGCAATTGCGAGCAGAGCTTTCAGGTACTACCTTACAGGGTGTCTCCAATAATAGGCTATATGTTGAGAAAGACAAG
AAAACTCTGTCCTTTGGACAGGTCAAAAGCCATGATTTTAGAAAAGCAGCTGGCAATGGTCGAGGCTATCAGCCCATGCT
GTTTGGCTTGTCAAGTAGCCAAATAACAACAGTAGGTCAGCAGGTTATCATCAAGCTGAAATGGCAAAGCGGCTTAGAAA
GGACCTTTATTTATGCATTTCAAGAGAAGGGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

52.083

100

0.521
  comYF Streptococcus mutans UA159

51.389

100

0.514
  comGF/cglF Streptococcus mitis SK321

50.365

95.139

0.479
  comGF/cglF Streptococcus mitis NCTC 12261

49.635

95.139

0.472
  comGF/cglF Streptococcus pneumoniae Rx1

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae D39

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae R6

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae TIGR4

48.175

95.139

0.458
  comGF Lactococcus lactis subsp. cremoris KW2

46.429

97.222

0.451

Multiple sequence alignment