Detailed information NTG0400439

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HUF09_RS02110
• Genome accession: NZ_CP054623
• Coordinates: 448299..449447 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain DL201330
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 443299..454447

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUF09_RS02085 (HUF09_02085)	-	444450..444746 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HUF09_RS02090 (HUF09_02090)	-	444743..445336 (-)	594	WP_003084544.1	YggT family protein	-
HUF09_RS02095 (HUF09_02095)	proC	445347..446168 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HUF09_RS02100 (HUF09_02100)	-	446180..446872 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
HUF09_RS02105 (HUF09_02105)	pilT	447087..448121 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HUF09_RS02110 (HUF09_02110)	pilU	448299..449447 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HUF09_RS02115 (HUF09_02115)	czcD	449455..450354 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HUF09_RS02120 (HUF09_02120)	-	450508..450912 (+)	405	WP_003084560.1	NINE protein	-
HUF09_RS02125 (HUF09_02125)	-	451155..452528 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HUF09_RS02130 (HUF09_02130)	-	452525..453709 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=400439 HUF09_RS02110 WP_003100959.1 448299..449447(+) (pilU) [Pseudomonas aeruginosa strain DL201330]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=400439 HUF09_RS02110 WP_003100959.1 448299..449447(+) (pilU) [Pseudomonas aeruginosa strain DL201330]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382