Detailed information NTG0400233

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HUF04_RS02100
• Genome accession: NZ_CP054581
• Coordinates: 448247..449395 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain YTSEY8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 443247..454395

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUF04_RS02075 (HUF04_02075)	-	444398..444694 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HUF04_RS02080 (HUF04_02080)	-	444691..445284 (-)	594	WP_003084544.1	YggT family protein	-
HUF04_RS02085 (HUF04_02085)	proC	445295..446116 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HUF04_RS02090 (HUF04_02090)	-	446128..446820 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HUF04_RS02095 (HUF04_02095)	pilT	447035..448069 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HUF04_RS02100 (HUF04_02100)	pilU	448247..449395 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HUF04_RS02105 (HUF04_02105)	czcD	449403..450302 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HUF04_RS02110 (HUF04_02110)	-	450456..450860 (+)	405	WP_003084560.1	NINE protein	-
HUF04_RS02115 (HUF04_02115)	-	451103..452476 (+)	1374	WP_034039850.1	pyridoxal-phosphate dependent enzyme	-
HUF04_RS02120 (HUF04_02120)	-	452473..453657 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=400233 HUF04_RS02100 WP_003100959.1 448247..449395(+) (pilU) [Pseudomonas aeruginosa strain YTSEY8]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=400233 HUF04_RS02100 WP_003100959.1 448247..449395(+) (pilU) [Pseudomonas aeruginosa strain YTSEY8]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382