Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GII77_RS13440 Genome accession   NZ_CP045825
Coordinates   2595126..2595743 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain 75     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2590126..2600743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII77_RS13410 (GII77_13410) rpsT 2590428..2590694 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  GII77_RS13415 (GII77_13415) holA 2590709..2591752 (-) 1044 WP_015251674.1 DNA polymerase III subunit delta -
  GII77_RS13420 (GII77_13420) - 2591792..2591941 (-) 150 WP_003229985.1 hypothetical protein -
  GII77_RS13425 (GII77_13425) yqzM 2591982..2592116 (+) 135 WP_003229983.1 YqzM family protein -
  GII77_RS13430 (GII77_13430) comEC 2592156..2594486 (-) 2331 WP_015251673.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GII77_RS13435 (GII77_13435) comEB 2594490..2595059 (-) 570 WP_003229978.1 ComE operon protein 2 -
  GII77_RS13440 (GII77_13440) comEA 2595126..2595743 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  GII77_RS13445 (GII77_13445) comER 2595827..2596648 (+) 822 WP_004398597.1 late competence protein ComER -
  GII77_RS13450 (GII77_13450) yqeM 2596714..2597457 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  GII77_RS13455 (GII77_13455) rsfS 2597454..2597810 (-) 357 WP_003229971.1 ribosome silencing factor -
  GII77_RS13460 (GII77_13460) yqeK 2597828..2598388 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GII77_RS13465 (GII77_13465) nadD 2598378..2598947 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GII77_RS13470 (GII77_13470) yhbY 2598959..2599249 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GII77_RS13475 (GII77_13475) aroE 2599243..2600085 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=398315 GII77_RS13440 WP_004398514.1 2595126..2595743(-) (comEA) [Bacillus subtilis strain 75]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=398315 GII77_RS13440 WP_004398514.1 2595126..2595743(-) (comEA) [Bacillus subtilis strain 75]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment