Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   TSC_RS12590 Genome accession   NC_014974
Coordinates   709752..710456 (-) Length   234 a.a.
NCBI ID   WP_081454906.1    Uniprot ID   A0A430RWU8
Organism   Thermus scotoductus SA-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 704752..715456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSC_RS03760 (TSC_c07680) trpS 705384..706397 (+) 1014 WP_015716675.1 tryptophan--tRNA ligase -
  TSC_RS03765 (TSC_c07690) - 706394..707095 (+) 702 WP_015716676.1 chromosome segregation protein ScpA -
  TSC_RS12585 (TSC_c07700) - 707150..707515 (+) 366 WP_015716677.1 nucleotidyltransferase family protein -
  TSC_RS13425 - 707500..707859 (-) 360 WP_041438121.1 type II secretion system protein -
  TSC_RS03780 (TSC_c07710) comZ 708078..709700 (-) 1623 WP_321162868.1 competence protein ComZ Machinery gene
  TSC_RS12590 pilA/pilA3 709752..710456 (-) 705 WP_081454906.1 PilW family protein Machinery gene
  TSC_RS12595 pilA/pilA2 710453..711013 (-) 561 WP_081454907.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  TSC_RS03785 (TSC_c07720) - 711010..711480 (-) 471 WP_041438123.1 GspH/FimT family protein -
  TSC_RS03790 (TSC_c07730) pilA/pilA1 711534..712004 (-) 471 WP_015716680.1 GspH/FimT family protein Machinery gene
  TSC_RS03795 (TSC_c07740) - 712075..713265 (+) 1191 WP_015716681.1 thiolase family protein -
  TSC_RS03800 (TSC_c07750) - 713366..713674 (-) 309 WP_015716682.1 DUF503 domain-containing protein -
  TSC_RS03805 (TSC_c07760) - 713731..714090 (-) 360 WP_015716683.1 DUF86 domain-containing protein -
  TSC_RS03810 (TSC_c07770) - 714087..714392 (-) 306 WP_015716684.1 nucleotidyltransferase family protein -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 25477.99 Da        Isoelectric Point: 5.0648

>NTDB_id=39732 TSC_RS12590 WP_081454906.1 709752..710456(-) (pilA/pilA3) [Thermus scotoductus SA-01]
MKGRGFTLIEVLVAIGLLAIILGVAVRYFINSSELARENQTRSELQDRVRMVMQVVSGDLQMAGARYWNSGDQNKGFSLP
AGKVLQGNNDGPKDTVSLYYVTSLRGKSEACRRVDYDFVNDTLRRSDVNATPTTGNDCTEPAPSYQPLAEGILALDIVYL
CSNGDRVNEPDCGADAYPRSAIVEVIGYSLNPMKAVGPQNLTTVSNEPLSCPVGRGCYALRQEVLMPNLKPLPQ

Nucleotide


Download         Length: 705 bp        

>NTDB_id=39732 TSC_RS12590 WP_081454906.1 709752..710456(-) (pilA/pilA3) [Thermus scotoductus SA-01]
GTGAAAGGAAGAGGCTTCACCCTAATAGAGGTGTTGGTAGCTATCGGCCTCCTGGCGATAATTTTGGGGGTCGCGGTGCG
GTATTTCATCAATAGCTCCGAGCTGGCCCGGGAAAACCAGACCAGAAGTGAACTGCAGGATCGGGTCCGTATGGTCATGC
AGGTAGTCAGTGGGGACCTACAAATGGCTGGGGCACGATACTGGAATAGCGGAGACCAGAACAAAGGCTTCTCCCTCCCT
GCAGGTAAAGTGCTACAGGGTAACAACGATGGCCCCAAGGACACCGTGAGCCTTTACTATGTCACTAGCCTGCGAGGAAA
AAGCGAAGCTTGTCGCCGTGTGGACTATGACTTTGTGAATGACACTCTTCGACGGAGTGACGTAAACGCCACTCCAACCA
CCGGAAACGACTGTACTGAACCTGCCCCTTCCTATCAGCCTTTGGCTGAAGGCATTCTTGCCTTAGACATAGTATACCTT
TGTAGCAATGGCGACAGAGTTAACGAACCCGATTGCGGTGCTGACGCCTATCCTCGCTCCGCCATTGTTGAAGTGATTGG
CTACTCTTTAAACCCAATGAAAGCAGTGGGGCCCCAAAACTTGACCACCGTGTCCAATGAACCCCTTTCTTGCCCTGTCG
GTCGGGGCTGTTATGCCCTCAGGCAAGAGGTTCTAATGCCGAACCTTAAGCCCCTGCCCCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A430RWU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

69.13

98.291

0.679

Multiple sequence alignment