Detailed information NTG0397149

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GFC07_RS19050
• Genome accession: NZ_CP045783
• Coordinates: 3992955..3993755 (-)
• Length: 266 a.a.
• NCBI ID: WP_023297075.1
• Uniprot ID: -
• Organism: Klebsiella variicola strain LEMB11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3987955..3998755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFC07_RS19030 (GFC07_19030)	dksA	3988536..3988991 (-)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-
GFC07_RS19035 (GFC07_19035)	sfsA	3989154..3989870 (-)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
GFC07_RS19040 (GFC07_19040)	thpR	3989870..3990406 (-)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
GFC07_RS19045 (GFC07_19045)	hrpB	3990479..3992908 (+)	2430	WP_153690898.1	ATP-dependent helicase HrpB	-
GFC07_RS19050 (GFC07_19050)	pilD	3992955..3993755 (-)	801	WP_023297075.1	A24 family peptidase	Machinery gene
GFC07_RS19055 (GFC07_19055)	-	3993752..3994498 (-)	747	WP_023297076.1	type II secretion system protein N	-
GFC07_RS19060 (GFC07_19060)	-	3994488..3994973 (-)	486	WP_012968921.1	type II secretion system protein M	-
GFC07_RS19065 (GFC07_19065)	gspL	3994966..3996162 (-)	1197	WP_153690899.1	type II secretion system protein GspL	-
GFC07_RS19070 (GFC07_19070)	gspK	3996155..3997135 (-)	981	WP_153690900.1	type II secretion system minor pseudopilin GspK	-
GFC07_RS19075 (GFC07_19075)	gspJ	3997132..3997728 (-)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
GFC07_RS19080 (GFC07_19080)	gspI	3997725..3998090 (-)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
GFC07_RS19085 (GFC07_19085)	gspH	3998087..3998602 (-)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29491.18 Da
• Isoelectric Point: 8.4742

>NTDB_id=397149 GFC07_RS19050 WP_023297075.1 3992955..3993755(-) (pilD) [Klebsiella variicola strain LEMB11]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIEWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=397149 GFC07_RS19050 WP_023297075.1 3992955..3993755(-) (pilD) [Klebsiella variicola strain LEMB11]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGAGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425