Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   GHB96_RS10530 Genome accession   NZ_CP045707
Coordinates   2027096..2027518 (-) Length   140 a.a.
NCBI ID   WP_215772521.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain NG250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2022096..2032518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB96_RS10510 (GHB96_11080) - 2022284..2023225 (-) 942 WP_003686969.1 ABC transporter ATP-binding protein -
  GHB96_RS10515 (GHB96_11085) - 2023234..2024109 (-) 876 WP_003686971.1 NUDIX domain-containing protein -
  GHB96_RS10525 (GHB96_11095) thiC 2024707..2026608 (+) 1902 WP_003686973.1 phosphomethylpyrimidine synthase ThiC -
  GHB96_RS10530 (GHB96_11100) pilE 2027096..2027518 (-) 423 WP_215772521.1 pilin Machinery gene
  GHB96_RS10535 (GHB96_11105) - 2027923..2028315 (-) 393 Protein_2052 pilin -
  GHB96_RS12020 - 2028477..2028827 (-) 351 Protein_2053 pilin -
  GHB96_RS12025 - 2028867..2029268 (-) 402 Protein_2054 pilin -
  GHB96_RS10545 (GHB96_11120) - 2029656..2030105 (-) 450 Protein_2055 pilin -
  GHB96_RS10550 (GHB96_11125) - 2030597..2030950 (-) 354 Protein_2056 pilin -
  GHB96_RS10555 (GHB96_11130) pilE 2031003..2031428 (-) 426 WP_050161409.1 pilin Machinery gene
  GHB96_RS10560 (GHB96_11135) - 2031480..2031788 (+) 309 Protein_2058 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15160.80 Da        Isoelectric Point: 5.9326

>NTDB_id=396183 GHB96_RS10530 WP_215772521.1 2027096..2027518(-) (pilE) [Neisseria gonorrhoeae strain NG250]
MPTDPDSRLRGNDEAILLAEGQKSAVTEYYLNNGEWPKNNDEAGVASSDKIKGKYVESVTVTNGVVTAKMKPSGVNKEIQ
GKRLSLWAKREDGSVKWFCGQPVTRGAGNAGKADDVTKDDTNAIETKHLPSTCRDNFDAS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=396183 GHB96_RS10530 WP_215772521.1 2027096..2027518(-) (pilE) [Neisseria gonorrhoeae strain NG250]
GTGCCGACAGACCCGGATTCCCGCCTGCGCGGGAATGACGAAGCCATCCTTTTGGCCGAAGGTCAAAAATCAGCCGTTAC
CGAGTATTACCTGAATAACGGCGAATGGCCGAAAAACAACGACGAGGCCGGCGTGGCATCCTCCGACAAAATCAAAGGCA
AATATGTTGAAAGCGTTACGGTCACAAACGGCGTCGTTACCGCCAAAATGAAACCAAGCGGCGTAAACAAAGAAATCCAA
GGCAAAAGACTCTCCCTGTGGGCCAAGCGTGAAGACGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTACGCGCGGCGC
CGGCAACGCCGGCAAAGCCGACGACGTCACCAAAGACGACACCAACGCCATCGAAACCAAGCACCTGCCGTCAACCTGCC
GCGATAACTTTGATGCCAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(14-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

65.957

100

0.664
  pilE Neisseria gonorrhoeae MS11

64.029

99.286

0.636

Multiple sequence alignment