Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   GHB96_RS10555 Genome accession   NZ_CP045707
Coordinates   2031003..2031428 (-) Length   141 a.a.
NCBI ID   WP_050161409.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain NG250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2026003..2036428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB96_RS10530 (GHB96_11100) pilE 2027096..2027518 (-) 423 WP_215772521.1 pilin Machinery gene
  GHB96_RS10535 (GHB96_11105) - 2027923..2028315 (-) 393 Protein_2052 pilin -
  GHB96_RS12020 - 2028477..2028827 (-) 351 Protein_2053 pilin -
  GHB96_RS12025 - 2028867..2029268 (-) 402 Protein_2054 pilin -
  GHB96_RS10545 (GHB96_11120) - 2029656..2030105 (-) 450 Protein_2055 pilin -
  GHB96_RS10550 (GHB96_11125) - 2030597..2030950 (-) 354 Protein_2056 pilin -
  GHB96_RS10555 (GHB96_11130) pilE 2031003..2031428 (-) 426 WP_050161409.1 pilin Machinery gene
  GHB96_RS10560 (GHB96_11135) - 2031480..2031788 (+) 309 Protein_2058 NAD(P)-dependent oxidoreductase -
  GHB96_RS10565 (GHB96_11140) metG 2031863..2033917 (-) 2055 WP_003686982.1 methionine--tRNA ligase -
  GHB96_RS10570 (GHB96_11145) glmS 2034033..2035871 (-) 1839 WP_047922618.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15292.38 Da        Isoelectric Point: 9.6170

>NTDB_id=396184 GHB96_RS10555 WP_050161409.1 2031003..2031428(-) (pilE) [Neisseria gonorrhoeae strain NG250]
MASASTIKGKYVQKVEVAKGVVTAQMASTGVNKEIKNKKLSLWARREAGSVKWFCGQPVTRDKAVTDDAVKDVTGNDKIE
TKHLPSTCRCFQIGSVKWFCGQPVTRDNAGTDAVTADTTGKDKEIDTKHLPSTCRDKSSAE

Nucleotide


Download         Length: 426 bp        

>NTDB_id=396184 GHB96_RS10555 WP_050161409.1 2031003..2031428(-) (pilE) [Neisseria gonorrhoeae strain NG250]
GTGGCATCCGCTTCAACAATCAAAGGCAAATATGTTCAGAAAGTTGAAGTCGCAAAAGGCGTCGTCACCGCCCAAATGGC
TTCAACCGGCGTAAACAAAGAAATCAAAAACAAAAAACTCTCCCTGTGGGCCAGGCGTGAAGCCGGTTCGGTAAAATGGT
TCTGCGGACAGCCGGTTACGCGCGACAAAGCCGTCACCGACGACGCCGTCAAAGACGTCACCGGCAACGACAAAATCGAA
ACCAAGCACCTGCCGTCAACCTGCCGTTGCTTTCAAATCGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTACGCGCGA
CAACGCCGGCACCGACGCCGTCACCGCCGACACCACCGGCAAAGACAAAGAAATCGACACCAAGCACCTGCCGTCAACCT
GCCGCGATAAATCATCTGCCGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(2-89)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

78.652

63.121

0.496
  pilE Neisseria gonorrhoeae MS11

69.88

58.865

0.411

Multiple sequence alignment