Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GFB47_RS01095 Genome accession   NZ_CP045699
Coordinates   245770..246834 (+) Length   354 a.a.
NCBI ID   WP_153445874.1    Uniprot ID   -
Organism   Vibrio algicola strain SM1977     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 240770..251834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB47_RS01080 (GFB47_01085) - 241057..241785 (-) 729 WP_153445869.1 glutathione peroxidase -
  GFB47_RS01085 (GFB47_01090) oxyR 242019..242903 (+) 885 WP_153445871.1 DNA-binding transcriptional regulator OxyR -
  GFB47_RS01090 (GFB47_01095) - 243037..245622 (-) 2586 WP_153445872.1 penicillin-binding protein 1A -
  GFB47_RS01095 (GFB47_01100) pilM 245770..246834 (+) 1065 WP_153445874.1 type IV pilus assembly protein PilM Machinery gene
  GFB47_RS01100 (GFB47_01105) pilN 246824..247411 (+) 588 WP_153445876.1 PilN domain-containing protein Machinery gene
  GFB47_RS01105 (GFB47_01110) pilO 247411..247998 (+) 588 WP_153445877.1 type 4a pilus biogenesis protein PilO Machinery gene
  GFB47_RS01110 (GFB47_01115) pilP 247995..248504 (+) 510 WP_153445880.1 pilus assembly protein PilP Machinery gene
  GFB47_RS01115 (GFB47_01120) pilQ 248542..250389 (+) 1848 WP_225874274.1 type IV pilus secretin PilQ Machinery gene
  GFB47_RS01120 (GFB47_01125) aroK 250767..251285 (+) 519 WP_153448097.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39526.31 Da        Isoelectric Point: 6.3461

>NTDB_id=396059 GFB47_RS01095 WP_153445874.1 245770..246834(+) (pilM) [Vibrio algicola strain SM1977]
MGKSFVTGIDIGHHSIKAVVLKSAGEQFELVSYKEIPVSEGIFAENHTFSHQGIVKKLKELKKQLPLMSRQVALALPDNA
VISKLLHIDGDLEDREKEFAIYQVFGQQSPFPIEDLSLDYVKVEQDSLKRGTTATYQVYATRKEVVESRVEVIKKSGFKP
VVIDLQAHSLLRVWQLAAQQQEDKQAWMMVDIGQSQTSICIIPQGRPAYYKDLPYGMSNMPEATQKLDLNVVEFEHGESV
IDLGKAQDSGIDHHEDEFIAELADRLTRQMSLYGSVNQQTKIEGLWLTGGGACISGITEALTQQLQIPVEVLNPFQLLES
KKNKKVSNLAPCTFSVAAGLAIRGLQWQRRIHVA

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=396059 GFB47_RS01095 WP_153445874.1 245770..246834(+) (pilM) [Vibrio algicola strain SM1977]
ATGGGTAAATCTTTTGTTACAGGCATTGATATTGGTCACCACAGCATTAAAGCAGTAGTACTAAAATCGGCCGGAGAACA
GTTCGAACTGGTGAGTTATAAAGAAATTCCAGTTTCGGAAGGCATTTTTGCAGAAAACCATACCTTTAGTCATCAGGGCA
TTGTTAAAAAATTAAAAGAGTTGAAGAAACAATTGCCGCTGATGTCGCGTCAGGTTGCTTTAGCGCTGCCAGATAATGCC
GTTATCAGTAAGTTATTGCATATTGACGGCGATCTTGAAGATCGCGAAAAAGAATTCGCCATTTACCAAGTGTTCGGCCA
GCAATCGCCATTTCCAATCGAAGATCTTAGCTTAGATTACGTCAAAGTAGAGCAAGACAGCTTAAAGCGTGGCACCACCG
CCACCTATCAAGTGTACGCCACGCGTAAAGAAGTGGTCGAAAGTCGAGTGGAGGTGATTAAAAAATCAGGCTTTAAACCA
GTGGTTATCGATCTGCAAGCGCATAGCTTATTGCGAGTGTGGCAATTAGCGGCGCAACAACAGGAGGATAAGCAAGCGTG
GATGATGGTCGATATTGGTCAATCTCAAACCTCGATCTGCATTATTCCGCAAGGCCGCCCGGCTTATTACAAAGACCTAC
CTTATGGCATGAGCAATATGCCAGAGGCGACGCAAAAACTTGATCTTAATGTGGTGGAGTTTGAACACGGCGAAAGCGTG
ATTGATTTAGGCAAGGCGCAAGACAGCGGGATTGATCACCATGAAGATGAATTTATTGCTGAGCTGGCCGACCGCCTGAC
GCGACAAATGAGTTTATATGGTTCAGTTAATCAGCAGACAAAAATTGAAGGATTGTGGTTGACGGGGGGCGGCGCGTGTA
TTTCGGGGATCACCGAAGCGTTAACCCAACAATTACAAATCCCGGTTGAAGTCCTAAACCCATTTCAATTACTCGAAAGC
AAAAAGAATAAAAAAGTTTCCAACTTAGCACCATGCACTTTTAGTGTGGCGGCCGGATTAGCCATACGCGGCCTGCAATG
GCAAAGGAGGATTCATGTTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

50.696

100

0.514
  pilM Vibrio campbellii strain DS40M4

43.875

99.153

0.435

Multiple sequence alignment