Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GFB47_RS01115 Genome accession   NZ_CP045699
Coordinates   248542..250389 (+) Length   615 a.a.
NCBI ID   WP_225874274.1    Uniprot ID   -
Organism   Vibrio algicola strain SM1977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 243542..255389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB47_RS01095 (GFB47_01100) pilM 245770..246834 (+) 1065 WP_153445874.1 type IV pilus assembly protein PilM Machinery gene
  GFB47_RS01100 (GFB47_01105) pilN 246824..247411 (+) 588 WP_153445876.1 PilN domain-containing protein Machinery gene
  GFB47_RS01105 (GFB47_01110) pilO 247411..247998 (+) 588 WP_153445877.1 type 4a pilus biogenesis protein PilO Machinery gene
  GFB47_RS01110 (GFB47_01115) pilP 247995..248504 (+) 510 WP_153445880.1 pilus assembly protein PilP Machinery gene
  GFB47_RS01115 (GFB47_01120) pilQ 248542..250389 (+) 1848 WP_225874274.1 type IV pilus secretin PilQ Machinery gene
  GFB47_RS01120 (GFB47_01125) aroK 250767..251285 (+) 519 WP_153448097.1 shikimate kinase AroK -
  GFB47_RS01125 (GFB47_01130) aroB 251310..252401 (+) 1092 WP_153445882.1 3-dehydroquinate synthase -
  GFB47_RS01130 (GFB47_01135) - 252478..253890 (+) 1413 WP_153445884.1 SPOR domain-containing protein -
  GFB47_RS01135 (GFB47_01140) - 254028..254867 (+) 840 WP_153445886.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 615 a.a.        Molecular weight: 66359.61 Da        Isoelectric Point: 4.8150

>NTDB_id=396063 GFB47_RS01115 WP_225874274.1 248542..250389(+) (pilQ) [Vibrio algicola strain SM1977]
MHKGITHTGLTCTGLNKAFSSLLNSLRICLLPVCLILVAQVASAAPASSIAANANKPLVKDNGLKNTFLGLDFRLNQQKE
AVIVVKLSSSVSAVDIKKNKEGLEIELIHTEVKDDDLYVIDVKDFSTAVSTVEVFRDNDNTRLQAHIRGEFTYDYRLKGK
QLEITVTEVAPEAAKKIKDSKGSLGADNKLISINFQDIPVRNVLQLIADYNKFNLVVSDSVTGNLTLRLDGVPWQQVLDI
ILQVKGLDKRVEGNVVLIAPKAEMDEQQKKRLEQARLQDELGDLASDLIQINFAKASDIADMIGGEGEVSMLSSRGNITV
DERTNSLLIRDLPSNIEIIKGIVESLDIPVKQVQIEARIVTINEGNIEDLGVRWGVASTNGNFSTGGSIESTTGQAGIAD
GRNGINFIPGQGDSTTGIDDFLNVNLAATSGNASSIAFQVAKLGSDTLLDLELSALQSENKAEIISSPRLITTNKQPAYI
EQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQITPDNRLVLDLSVTQDRPGAVVKTGDGEAVAIDTQRIGTQVLVNNGE
TVVLGGIYQHSVQKSVDKVPLLGDIPYLGALFRRSYENYGKSELLIFVTPKVILQ

Nucleotide


Download         Length: 1848 bp        

>NTDB_id=396063 GFB47_RS01115 WP_225874274.1 248542..250389(+) (pilQ) [Vibrio algicola strain SM1977]
ATGCATAAAGGAATAACGCATACAGGATTAACGTGCACCGGATTGAATAAAGCGTTCTCATCGCTATTGAATAGCTTAAG
AATCTGTTTGTTGCCGGTTTGTTTGATATTAGTCGCTCAAGTCGCCTCTGCTGCGCCAGCCTCAAGCATTGCGGCCAATG
CCAATAAACCGTTGGTCAAAGATAATGGATTGAAAAATACATTCTTAGGCTTGGATTTTCGGTTAAACCAGCAAAAAGAA
GCAGTGATAGTGGTTAAATTATCCTCGTCAGTGTCAGCGGTGGATATTAAGAAGAACAAAGAAGGTTTAGAAATTGAGTT
AATCCATACAGAGGTTAAAGATGATGATCTTTATGTGATTGATGTAAAAGATTTCTCTACCGCAGTAAGCACGGTGGAAG
TGTTCCGCGATAATGATAACACTCGTTTGCAAGCTCATATCCGTGGCGAGTTTACTTATGATTACCGGTTGAAGGGGAAG
CAGCTTGAAATTACCGTCACCGAAGTCGCCCCAGAAGCGGCTAAAAAAATCAAAGACAGTAAAGGTTCGCTCGGGGCGGA
TAACAAGTTAATCTCAATCAACTTCCAAGACATTCCAGTGCGTAATGTCTTGCAGTTGATTGCCGATTACAACAAATTTA
ACTTGGTAGTTTCTGATTCAGTGACGGGTAATTTAACCTTACGTCTGGATGGCGTTCCTTGGCAGCAAGTGTTAGATATT
ATCTTGCAAGTCAAAGGGCTAGATAAACGAGTGGAAGGGAATGTGGTATTAATTGCGCCCAAAGCTGAAATGGATGAGCA
ACAAAAGAAGCGTTTAGAGCAAGCGCGTTTACAAGATGAACTGGGTGATTTAGCGTCCGACCTTATACAAATCAATTTTG
CTAAAGCCTCAGATATTGCTGACATGATTGGCGGTGAAGGTGAAGTGAGTATGCTATCAAGCCGTGGAAATATTACGGTT
GATGAGCGGACTAACTCATTATTAATTCGTGATTTACCCTCTAATATCGAAATTATCAAAGGCATTGTTGAGTCTTTAGA
TATTCCTGTTAAACAAGTGCAGATCGAAGCGCGCATTGTGACGATCAATGAAGGTAATATCGAAGACTTAGGGGTTCGTT
GGGGCGTCGCGAGTACCAATGGTAACTTTTCGACTGGTGGCAGTATAGAAAGTACTACGGGGCAAGCTGGTATCGCCGAT
GGCCGAAACGGGATCAATTTTATTCCAGGTCAAGGTGATTCAACGACTGGTATCGACGACTTCTTGAACGTTAACTTAGC
AGCGACAAGTGGTAATGCTTCAAGTATTGCATTCCAAGTAGCTAAATTGGGCTCCGATACATTACTGGATTTAGAATTAT
CAGCGTTACAAAGTGAGAATAAAGCGGAAATAATTTCAAGCCCACGTTTGATCACCACCAATAAACAACCTGCTTATATT
GAGCAAGGCACTGAAATTCCTTATTTAGAGGCTTCTTCTAGTGGTGCGACATCGGTTAGCTTTAAGAAAGCAGTACTGAG
CTTAATGGTAACGCCACAAATCACACCCGATAACCGTTTGGTGCTTGATTTAAGTGTTACTCAAGATAGGCCAGGAGCGG
TAGTGAAAACCGGTGATGGTGAAGCGGTGGCGATTGATACTCAGCGTATCGGTACTCAGGTACTGGTTAACAATGGCGAA
ACTGTGGTACTCGGTGGTATTTATCAACACTCGGTACAAAAAAGTGTCGATAAAGTGCCACTACTGGGCGACATTCCTTA
TTTAGGCGCGTTGTTCCGTCGTAGCTATGAAAACTACGGTAAGAGCGAGTTGTTGATATTTGTTACCCCAAAAGTGATCT
TGCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(455-613)

Bacterial type II/III secretion system short domain (Secretin_N)

(288-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

72.727

89.431

0.65
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

68.739

94.146

0.647
  pilQ Vibrio cholerae strain A1552

68.739

94.146

0.647
  pilQ Pseudomonas aeruginosa PAK

40.137

94.797

0.38
  pilQ Legionella pneumophila strain ERS1305867

41.667

89.756

0.374
  pilQ Legionella pneumophila strain Lp02

41.667

89.756

0.374
  pilQ Acinetobacter baumannii strain A118

36.738

98.699

0.363
  pilQ Acinetobacter baumannii D1279779

36.738

98.699

0.363

Multiple sequence alignment