Detailed information NTG0394974

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GAY96_RS09715
• Genome accession: NZ_CP045550
• Coordinates: 2049234..2050412 (+)
• Length: 392 a.a.
• NCBI ID: WP_265573360.1
• Uniprot ID: -
• Organism: Venatoribacter cucullus strain ASxS5
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2044234..2055412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GAY96_RS09690 (GAY96_09630)	rplS	2044842..2045195 (-)	354	WP_228344803.1	50S ribosomal protein L19	-
GAY96_RS09695 (GAY96_09635)	trmD	2045206..2045961 (-)	756	WP_265573358.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
GAY96_RS09700 (GAY96_09640)	rimM	2045965..2046501 (-)	537	WP_228344805.1	ribosome maturation factor RimM	-
GAY96_RS09705 (GAY96_09645)	rpsP	2046523..2046774 (-)	252	WP_228344806.1	30S ribosomal protein S16	-
GAY96_RS09710 (GAY96_09650)	-	2047107..2048843 (+)	1737	WP_265573359.1	Na/Pi cotransporter family protein	-
GAY96_RS09715 (GAY96_09655)	pilU	2049234..2050412 (+)	1179	WP_265573360.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GAY96_RS09720 (GAY96_09660)	purL	2050528..2054421 (-)	3894	WP_265573361.1	phosphoribosylformylglycinamidine synthase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 42851.01 Da
• Isoelectric Point: 7.3519

>NTDB_id=394974 GAY96_RS09715 WP_265573360.1 2049234..2050412(+) (pilU) [Venatoribacter cucullus strain ASxS5]
MSIENWLHLLAKHDGSDLYLSKGAPPCAKFQGQLKHLSRDVLGPGDIEKIAYSIMSDAQRKEFEEEFELNMAMSNSSGRF
RINIFKQQHEVSIVARNINTEIPDIAKLNLPPVLKDVITAKRGLILFVGGTGSGKSTSLAALIDHRNATTAGHIITIEDP
IEFVHKHKKSVINQREVGTDTKSYAAALKNTLRQAPDVILIGEIRDAETMEHALAFAETGHLAISTLHANNANQAFERIV
NMFPEERRNQILLGLGQNVRAIISQRLIPTVDGKRAAAVEVLLGNKTVEDLIIRGDFPSIKEIMSKSENLGMQTFDGALY
HLYKQGKITLEQALKNADSENNLRLRIKLGDNKPAAKPAESATKPAAPKAAAATSGLSLSLVEEGSVEEAYA

Nucleotide

• Length: 1179 bp

>NTDB_id=394974 GAY96_RS09715 WP_265573360.1 2049234..2050412(+) (pilU) [Venatoribacter cucullus strain ASxS5]
ATGAGCATCGAAAACTGGTTGCATCTACTGGCAAAACACGATGGCTCAGACCTCTACCTGAGCAAAGGTGCACCGCCCTG
TGCGAAATTTCAGGGGCAATTAAAGCACCTCAGCCGCGACGTACTTGGCCCCGGCGATATTGAAAAAATCGCCTATTCCA
TTATGTCGGACGCGCAACGCAAAGAATTTGAAGAAGAATTCGAACTGAACATGGCCATGTCTAATTCGTCCGGCCGTTTC
CGTATTAATATTTTCAAGCAACAGCACGAAGTGTCGATCGTTGCCCGCAACATCAACACCGAAATTCCGGACATTGCCAA
ACTGAACCTGCCGCCGGTCTTAAAAGACGTAATTACCGCCAAGCGCGGCCTGATTCTGTTTGTCGGTGGTACCGGCTCGG
GTAAATCCACCTCGCTGGCAGCACTGATCGACCACCGTAATGCCACCACCGCCGGCCATATTATTACCATCGAAGACCCG
ATTGAATTTGTGCACAAGCACAAGAAAAGCGTGATTAACCAGCGCGAAGTAGGCACCGACACCAAGAGCTACGCCGCCGC
ATTAAAAAACACCCTGCGTCAGGCGCCGGACGTGATTTTAATTGGCGAAATCCGCGACGCGGAAACCATGGAGCATGCCC
TCGCCTTTGCCGAAACCGGCCACCTGGCGATTTCTACCCTGCACGCCAACAACGCCAACCAGGCGTTTGAACGCATTGTG
AATATGTTCCCGGAAGAGCGCCGCAACCAGATTCTGCTGGGCCTGGGCCAAAACGTGCGCGCCATTATTTCCCAGCGTTT
AATTCCCACTGTTGATGGCAAACGTGCCGCCGCCGTGGAAGTGCTGCTGGGCAATAAAACCGTGGAAGACCTGATTATCC
GCGGCGATTTTCCATCCATTAAAGAAATCATGAGCAAATCCGAAAACCTCGGCATGCAGACCTTCGATGGCGCGCTCTAC
CACCTGTACAAACAGGGTAAGATCACACTGGAACAGGCGCTGAAAAACGCCGACTCGGAAAACAACCTGCGCTTGCGCAT
TAAACTGGGCGATAACAAACCGGCAGCCAAACCCGCTGAGTCGGCTACAAAACCAGCCGCACCGAAAGCAGCTGCAGCAA
CATCCGGGCTGTCATTGAGCCTGGTGGAAGAAGGTTCTGTGGAAGAAGCCTACGCCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (54-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	52.646	91.582	0.482
pilU	Pseudomonas stutzeri DSM 10701	53.125	89.796	0.477
pilU	Vibrio cholerae strain A1552	49.284	89.031	0.439
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.82	97.704	0.418