Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSESU_RS03315 Genome accession   NC_014924
Coordinates   745487..747217 (+) Length   576 a.a.
NCBI ID   WP_013534353.1    Uniprot ID   E6WQS4
Organism   Pseudoxanthomonas suwonensis 11-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 733331..761043 745487..747217 within 0


Gene organization within MGE regions


Location: 733331..761043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSESU_RS03260 (Psesu_0656) - 734988..736784 (+) 1797 WP_233275249.1 ABC transporter ATP-binding protein -
  PSESU_RS03265 (Psesu_0657) - 736781..737533 (+) 753 WP_013534343.1 class I SAM-dependent methyltransferase -
  PSESU_RS03270 (Psesu_0658) - 737530..738132 (+) 603 WP_013534344.1 HAD family phosphatase -
  PSESU_RS03275 (Psesu_0659) - 738183..738794 (+) 612 WP_013534345.1 NTP transferase domain-containing protein -
  PSESU_RS03280 (Psesu_0660) - 738804..739697 (+) 894 WP_013534346.1 LicD family protein -
  PSESU_RS03285 (Psesu_0661) - 739694..740956 (+) 1263 WP_013534347.1 CDP-glycerol--poly(glycerophosphate) glycerophosphotransferase -
  PSESU_RS03290 (Psesu_0662) - 740986..741372 (+) 387 WP_041763803.1 adenylyltransferase/cytidyltransferase family protein -
  PSESU_RS03295 (Psesu_0663) - 741369..742544 (-) 1176 WP_013534349.1 CDP-glycerol--glycerophosphate glycerophosphotransferase -
  PSESU_RS03300 (Psesu_0664) - 742541..743629 (-) 1089 WP_081459335.1 glycosyltransferase family 4 protein -
  PSESU_RS03305 (Psesu_0665) - 743704..744570 (-) 867 WP_013534351.1 glycosyltransferase family 2 protein -
  PSESU_RS03310 (Psesu_0666) - 744662..745333 (-) 672 WP_013534352.1 hypothetical protein -
  PSESU_RS03315 (Psesu_0667) pilB 745487..747217 (+) 1731 WP_013534353.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSESU_RS03320 (Psesu_0668) - 747632..748111 (+) 480 WP_013534354.1 transposase -
  PSESU_RS16580 (Psesu_0669) - 748154..748438 (+) 285 WP_324603281.1 hypothetical protein -
  PSESU_RS03325 (Psesu_0670) ybgC 748898..749308 (+) 411 WP_013534355.1 tol-pal system-associated acyl-CoA thioesterase -
  PSESU_RS03330 (Psesu_0671) tolQ 749379..750155 (+) 777 WP_013534356.1 protein TolQ -
  PSESU_RS03335 (Psesu_0672) tolR 750181..750609 (+) 429 WP_013534357.1 protein TolR -
  PSESU_RS03340 (Psesu_0673) - 750629..751612 (+) 984 WP_013534358.1 cell envelope integrity protein TolA -
  PSESU_RS03345 (Psesu_0674) tolB 751833..753152 (+) 1320 WP_041763808.1 Tol-Pal system beta propeller repeat protein TolB -
  PSESU_RS03350 (Psesu_0675) pal 753224..753736 (+) 513 WP_013534360.1 peptidoglycan-associated lipoprotein Pal -
  PSESU_RS03355 (Psesu_0676) ybgF 753768..754547 (+) 780 WP_041764394.1 tol-pal system protein YbgF -
  PSESU_RS03360 (Psesu_0677) queE 754716..755375 (+) 660 WP_013534362.1 7-carboxy-7-deazaguanine synthase QueE -
  PSESU_RS03365 (Psesu_0678) queC 755372..756097 (+) 726 WP_267878632.1 7-cyano-7-deazaguanine synthase QueC -
  PSESU_RS03375 (Psesu_0679) - 756435..757091 (+) 657 WP_013534364.1 hypothetical protein -
  PSESU_RS16085 - 757091..757672 (+) 582 WP_155942703.1 hypothetical protein -
  PSESU_RS15855 (Psesu_0680) - 757635..758732 (+) 1098 WP_013534365.1 MBL fold metallo-hydrolase -
  PSESU_RS03385 (Psesu_0681) - 758729..758992 (-) 264 WP_155942704.1 integrase -
  PSESU_RS16090 - 759953..760462 (+) 510 WP_155942705.1 hypothetical protein -
  PSESU_RS03390 (Psesu_0683) - 760513..760695 (+) 183 WP_013534366.1 hypothetical protein -
  PSESU_RS16095 (Psesu_0684) - 760825..761043 (-) 219 WP_013534367.1 hypothetical protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62509.66 Da        Isoelectric Point: 5.3509

>NTDB_id=39483 PSESU_RS03315 WP_013534353.1 745487..747217(+) (pilB) [Pseudoxanthomonas suwonensis 11-1]
MNVATTANLVGITGIARRLVQDGAMDEGAARTAMAQAAEAKVPLAQFIADKKLVSPAQLAAANSVEFGIPLLDVTAFDAS
QNAMKLVSEELLQKHRLLPLFKRGSKLFVGVSSPTQTGALAEIQFHTNLAVEPILVDEEQIRRTFEQWQNANAQLEGGLD
EDDEGLEGLEVSAGDEDGAGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKTVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCTKCKRAVDLPRN
ALLAEGFTEEEIDAGFTVYEPVGCDECTEGYKGRTGIYQVMPMSEEIQAIVLSGGNAMQIAEVAEKAGIADLRKSALKKV
REGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=39483 PSESU_RS03315 WP_013534353.1 745487..747217(+) (pilB) [Pseudoxanthomonas suwonensis 11-1]
ATGAACGTCGCCACCACCGCCAACCTTGTCGGTATCACCGGCATTGCCCGCCGTCTCGTCCAGGACGGCGCCATGGACGA
GGGTGCGGCCCGGACGGCCATGGCCCAGGCGGCCGAGGCCAAGGTGCCGCTGGCCCAGTTCATCGCCGACAAGAAGCTGG
TCAGCCCGGCCCAGCTGGCGGCCGCCAACTCGGTCGAGTTCGGCATCCCGCTGCTGGATGTGACCGCGTTCGACGCCTCC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGCTGCTGCAGAAGCACCGCCTGCTGCCCCTGTTCAAGCGCGGCAGCAAGCT
GTTCGTGGGCGTCAGCAGCCCCACCCAGACTGGCGCCCTGGCCGAAATCCAGTTCCACACCAACCTGGCCGTCGAGCCGA
TCCTGGTGGACGAGGAACAGATCCGCCGCACCTTCGAGCAGTGGCAGAACGCCAATGCCCAGCTCGAGGGCGGCCTTGAC
GAGGACGACGAGGGCCTGGAGGGGCTGGAAGTCAGTGCGGGCGACGAGGACGGGGCTGGTGACTCCGGCGTCGACGCCAA
GGGCGACGACACGCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCCGTGGCGCCTCGGACATCCACT
TCGAGCCCTACGAGACCGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTCAAGACCGTGGCCCGGGCGCCGGTGAAG
CTCAACCAGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGACGCGATCCACAAGCCCTACGGCATGGTCCTGGTCACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGACCCGGTCGAAATCCGGCTGCCCGGCGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCGCCGCAGACAATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGT
CGGTGACCCTGGTCATCGCCCAGCGCCTGGCACGCCGCCTGTGCACCAAGTGCAAGCGCGCGGTCGACCTGCCGCGCAAC
GCGCTGCTGGCCGAAGGCTTCACCGAGGAAGAGATCGATGCCGGGTTCACCGTGTACGAGCCGGTCGGCTGCGACGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGCATCTACCAGGTCATGCCGATGAGCGAGGAGATCCAGGCCATCGTGCTGTCCG
GCGGCAATGCCATGCAGATCGCCGAGGTCGCCGAGAAGGCCGGCATCGCCGACCTGCGCAAGTCGGCGCTGAAGAAGGTC
CGCGAAGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6WQS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.654

98.264

0.547
  pilB Acinetobacter baylyi ADP1

55.3

98.264

0.543
  pilB Legionella pneumophila strain ERS1305867

52.807

98.958

0.523
  pilF Neisseria gonorrhoeae MS11

49.47

98.264

0.486
  pilB Vibrio cholerae strain A1552

53.113

89.236

0.474
  pilB Vibrio campbellii strain DS40M4

46.221

98.785

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

46.346

97.396

0.451
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.919

100

0.37

Multiple sequence alignment