Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HPY13_RS00835 Genome accession   NZ_CP053796
Coordinates   177300..178811 (+) Length   503 a.a.
NCBI ID   WP_175249700.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL4G     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 172300..183811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY13_RS00815 (HPY13_00815) hslO 172800..173675 (-) 876 WP_001911276.1 Hsp33 family molecular chaperone HslO -
  HPY13_RS00820 (HPY13_00820) hslR 173709..174086 (-) 378 WP_000098851.1 ribosome-associated heat shock protein Hsp15 -
  HPY13_RS00825 (HPY13_00825) gspC 174316..175233 (+) 918 WP_057563696.1 type II secretion system protein GspC -
  HPY13_RS00830 (HPY13_00830) gspD 175279..177303 (+) 2025 WP_000878557.1 type II secretion system secretin GspD -
  HPY13_RS00835 (HPY13_00835) pilB/pilB1 177300..178811 (+) 1512 WP_175249700.1 type II secretion system ATPase GspE Machinery gene
  HPY13_RS00840 (HPY13_00840) gspF 178811..180031 (+) 1221 WP_000718695.1 type II secretion system inner membrane protein GspF -
  HPY13_RS00845 (HPY13_00845) gspG 180072..180512 (+) 441 WP_001897706.1 type II secretion system major pseudopilin GspG -
  HPY13_RS00850 (HPY13_00850) gspH 180546..181130 (+) 585 WP_175249701.1 type II secretion system minor pseudopilin GspH -
  HPY13_RS00855 (HPY13_00855) gspI 181120..181473 (+) 354 WP_000837699.1 type II secretion system minor pseudopilin GspI -
  HPY13_RS00860 (HPY13_00860) gspJ 181460..182125 (+) 666 WP_000269336.1 type II secretion system minor pseudopilin GspJ -
  HPY13_RS00865 (HPY13_00865) gspK 182115..183125 (+) 1011 WP_001201881.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56388.66 Da        Isoelectric Point: 6.2107

>NTDB_id=394762 HPY13_RS00835 WP_175249700.1 177300..178811(+) (pilB/pilB1) [Vibrio cholerae strain SL4G]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRAMGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=394762 HPY13_RS00835 WP_175249700.1 177300..178811(+) (pilB/pilB1) [Vibrio cholerae strain SL4G]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCGTTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGATTCTTCAGAAGCGCGTCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCGGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGTGAAG
CGATAAAAGAAGGCGCGTCAGATATTCACATTGAAACTTTTGAAAAAACACTGTCGATCCGTTTTCGAGTCGATGGCGTG
CTGCGCGAAGTACTGGCACCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAAGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGTGTGCCGCAAGATGGCCGTATTTCGCTGCGTATCGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGCCCAACCGG
CTCGGGTAAATCGACCACCTTGTACGCCGGTTTGCAAGAGCTCAACAGCAGCGAGCGCAACATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCAAGGGTAGACATGACCTTCGCGCGGGGGCTACGC
GCTATTTTGCGTCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGTGACTTGGAAACGGCGCAAATTGCAGTGCAAGC
CTCGCTGACCGGTCACTTAGTGATGTCGACTCTGCACACCAACACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCCTCTTCACTGCTCGGTGTTCTGGCTCAGCGCTTGGTGCGCACCTTATGCCCAGATTGC
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGCAAGAAAAAAGAACCGCTGATCCTTTATCGCGC
CATGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGCTGCTGGTGGATGATGCGT
TGCAGGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTCTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.49

77.932

0.386
  pilF Thermus thermophilus HB27

48.01

79.92

0.384
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376