Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   GAY69_RS13450 Genome accession   NZ_CP045472
Coordinates   2754837..2755334 (+) Length   165 a.a.
NCBI ID   WP_001838632.1    Uniprot ID   A0A6A9GZK7
Organism   Staphylococcus aureus strain ZY05     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2749837..2760334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GAY69_RS13410 (GAY69_13665) - 2749881..2750867 (+) 987 WP_000161315.1 ROK family glucokinase -
  GAY69_RS13415 (GAY69_13670) - 2750867..2751196 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  GAY69_RS13420 (GAY69_13675) - 2751193..2751816 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  GAY69_RS13425 (GAY69_13680) comGA 2751868..2752842 (+) 975 WP_000697218.1 competence type IV pilus ATPase ComGA Machinery gene
  GAY69_RS13430 (GAY69_13685) comGB 2752814..2753884 (+) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  GAY69_RS13435 (GAY69_13690) comGC 2753898..2754209 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  GAY69_RS13440 (GAY69_13695) comGD 2754187..2754633 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  GAY69_RS13445 (GAY69_13700) comGE 2754620..2754919 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  GAY69_RS13450 (GAY69_13705) comGF 2754837..2755334 (+) 498 WP_001838632.1 competence type IV pilus minor pilin ComGF Machinery gene
  GAY69_RS13455 (GAY69_13710) - 2755431..2755577 (+) 147 WP_001789879.1 hypothetical protein -
  GAY69_RS13460 (GAY69_13715) - 2755567..2756091 (+) 525 WP_154285237.1 shikimate kinase -
  GAY69_RS13465 (GAY69_13720) gcvT 2756250..2757341 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  GAY69_RS13470 (GAY69_13725) gcvPA 2757361..2758707 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  GAY69_RS13475 (GAY69_13730) gcvPB 2758700..2760172 (+) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19184.87 Da        Isoelectric Point: 10.2192

>NTDB_id=394449 GAY69_RS13450 WP_001838632.1 2754837..2755334(+) (comGF) [Staphylococcus aureus strain ZY05]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIENREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=394449 GAY69_RS13450 WP_001838632.1 2754837..2755334(+) (comGF) [Staphylococcus aureus strain ZY05]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCTCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAAATAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A9GZK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

96.364

100

0.964
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment