Detailed information NTG0394103

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HJ571_RS02450
• Genome accession: NZ_CP053747
• Coordinates: 527236..528384 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Pae1255-NDM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 522236..533384

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HJ571_RS02425 (HJ571_02435)	-	523387..523683 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HJ571_RS02430 (HJ571_02440)	-	523680..524273 (-)	594	WP_003084544.1	YggT family protein	-
HJ571_RS02435 (HJ571_02445)	proC	524284..525105 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
HJ571_RS02440 (HJ571_02450)	-	525117..525809 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HJ571_RS02445 (HJ571_02455)	pilT	526024..527058 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HJ571_RS02450 (HJ571_02460)	pilU	527236..528384 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HJ571_RS02455 (HJ571_02465)	czcD	528392..529291 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HJ571_RS02460 (HJ571_02470)	-	529446..529850 (+)	405	WP_003084560.1	NINE protein	-
HJ571_RS02465 (HJ571_02475)	-	530093..531466 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
HJ571_RS02470 (HJ571_02480)	-	531463..532647 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=394103 HJ571_RS02450 WP_003100959.1 527236..528384(+) (pilU) [Pseudomonas aeruginosa strain Pae1255-NDM1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=394103 HJ571_RS02450 WP_003100959.1 527236..528384(+) (pilU) [Pseudomonas aeruginosa strain Pae1255-NDM1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382