Detailed information NTG0393869

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FIU84_RS19855
• Genome accession: NZ_CP045416
• Coordinates: 4201573..4202718 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain THAF7b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4196573..4207718

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU84_RS19830 (FIU84_19870)	-	4196653..4197669 (+)	1017	WP_063540691.1	aspartate carbamoyltransferase catalytic subunit	-
FIU84_RS19835 (FIU84_19875)	-	4197669..4198943 (+)	1275	WP_110778830.1	dihydroorotase	-
FIU84_RS19840 (FIU84_19880)	-	4199000..4199395 (-)	396	WP_017245432.1	TM2 domain-containing protein	-
FIU84_RS19845 (FIU84_19885)	-	4199528..4200742 (+)	1215	WP_152481925.1	putative DNA modification/repair radical SAM protein	-
FIU84_RS19850 (FIU84_19890)	-	4200742..4201566 (+)	825	WP_152481926.1	TIGR03915 family putative DNA repair protein	-
FIU84_RS19855 (FIU84_19895)	pilU	4201573..4202718 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FIU84_RS19860 (FIU84_19900)	pilT	4202779..4203813 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
FIU84_RS19865 (FIU84_19905)	-	4203880..4204569 (+)	690	WP_063540687.1	YggS family pyridoxal phosphate-dependent enzyme	-
FIU84_RS19870 (FIU84_19910)	proC	4204580..4205404 (+)	825	WP_063540686.1	pyrroline-5-carboxylate reductase	-
FIU84_RS19875 (FIU84_19915)	-	4205414..4206004 (+)	591	WP_017245439.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=393869 FIU84_RS19855 WP_026006585.1 4201573..4202718(-) (pilU) [Stutzerimonas frequens strain THAF7b]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=393869 FIU84_RS19855 WP_026006585.1 4201573..4202718(-) (pilU) [Stutzerimonas frequens strain THAF7b]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATTACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGCGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAAAATCACGAATGCAACTTCGCCATCAGTGCCCGCGGGATCGGTCGC
TTCCGTGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATTCCGACCATCGA
AGAGCTGAAGCTGCCTGACGTTCTCAAGAAGCTGTCGATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAGACCGGGCACCTGTGTCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAACA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTGTTGATCAACACCCCTCTGGCCGCAGACCTTA
TCCGCAAGGGCGAGGTGCATGAACTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTATGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCACTGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388