Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FIU95_RS01050 Genome accession   NZ_CP045369
Coordinates   226363..227439 (+) Length   358 a.a.
NCBI ID   WP_152450675.1    Uniprot ID   A0A5P9ERM4
Organism   Microbulbifer sp. THAF38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 221363..232439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS01040 (FIU95_01045) - 222896..223378 (+) 483 WP_152450671.1 chemotaxis protein CheW -
  FIU95_RS01045 (FIU95_01050) - 223525..226215 (-) 2691 WP_152450673.1 penicillin-binding protein 1A -
  FIU95_RS01050 (FIU95_01055) pilM 226363..227439 (+) 1077 WP_152450675.1 pilus assembly protein PilM Machinery gene
  FIU95_RS01055 (FIU95_01060) - 227441..228016 (+) 576 WP_152450677.1 PilN domain-containing protein -
  FIU95_RS01060 (FIU95_01065) - 228028..228648 (+) 621 WP_152450679.1 type 4a pilus biogenesis protein PilO -
  FIU95_RS01065 (FIU95_01070) - 228645..229172 (+) 528 WP_152450681.1 pilus assembly protein PilP -
  FIU95_RS01070 (FIU95_01075) pilQ 229172..231361 (+) 2190 WP_152450683.1 type IV pilus secretin PilQ -
  FIU95_RS01075 (FIU95_01080) aroK 231358..231882 (+) 525 WP_256366399.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38896.18 Da        Isoelectric Point: 4.3421

>NTDB_id=393697 FIU95_RS01050 WP_152450675.1 226363..227439(+) (pilM) [Microbulbifer sp. THAF38]
MGIFPFLDKGPKAMLGLDISSTAVKLLELSRNGDKYRVESYAVEPLPPNAVVDKNINDIGATAEAIRKVVRRSKTRLRRA
AVAVSGSAVITKTLEMPADLSDEDLEARIALEADQYIPYPLEEVSLDFEVQGPSEKGDDQVEVLLAACRSENIEQRVSAL
GEAELEAGVVDVEAYAIERTYTLLEGQFPPQEQLVVAVVDIGATMSALSVLVDGKTVYTREQLFGGRQLTDEIQRRYGLS
AEEASLAKHQGGSGLPDDYFAEVLEPFRDAVIQQVTRALQFFYSSTSYSDVDYILLSGGVAAMEGLSDLVGEKLGKPTVV
ANPFFAMGTSSRVNKQMLAKEAPGLMIATGLAMREKEY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=393697 FIU95_RS01050 WP_152450675.1 226363..227439(+) (pilM) [Microbulbifer sp. THAF38]
ATGGGGATTTTCCCTTTTCTGGATAAAGGCCCGAAGGCCATGTTGGGACTGGATATTAGCTCGACCGCAGTAAAGTTGCT
GGAGCTAAGTCGCAATGGCGATAAATATCGAGTCGAAAGTTACGCCGTGGAACCTCTGCCGCCGAATGCGGTTGTGGACA
AGAACATCAATGATATTGGTGCCACGGCGGAAGCGATCCGCAAAGTCGTGCGCCGTTCCAAGACTCGCCTGCGCCGCGCA
GCTGTAGCCGTATCCGGCTCCGCGGTGATCACCAAAACCCTGGAAATGCCCGCTGACCTCTCTGATGAAGATTTGGAAGC
GCGTATTGCCCTGGAAGCGGACCAATACATTCCCTATCCACTTGAAGAAGTGAGCCTTGATTTTGAAGTGCAGGGACCTT
CTGAGAAGGGTGATGATCAGGTGGAAGTGCTGCTGGCAGCGTGCCGTAGCGAAAACATCGAGCAACGGGTTTCAGCCCTC
GGTGAGGCCGAGCTGGAGGCCGGGGTCGTCGATGTTGAAGCCTATGCCATTGAGCGCACTTACACTTTGCTGGAGGGTCA
GTTTCCTCCCCAAGAGCAGTTGGTTGTTGCGGTAGTGGATATCGGTGCGACCATGAGTGCTTTGTCCGTATTGGTAGATG
GCAAAACGGTTTATACCCGTGAACAGCTGTTCGGTGGTCGCCAGCTCACCGATGAGATACAGCGGCGCTATGGCCTCTCC
GCCGAAGAGGCATCTCTGGCGAAGCATCAGGGTGGCAGTGGTCTACCAGATGACTACTTTGCTGAGGTATTGGAACCGTT
CCGCGATGCAGTCATTCAACAAGTCACCCGTGCACTGCAGTTCTTTTATTCTTCGACTTCCTACAGCGATGTGGATTACA
TATTGCTCAGCGGTGGTGTTGCCGCTATGGAAGGGCTTTCTGATCTGGTGGGTGAAAAACTGGGGAAACCCACAGTGGTC
GCCAACCCATTCTTCGCCATGGGCACGTCTTCGCGAGTGAATAAGCAAATGCTTGCCAAAGAGGCTCCGGGACTGATGAT
CGCCACAGGGCTCGCCATGCGGGAAAAGGAGTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9ERM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.867

96.648

0.492
  comM Acinetobacter nosocomialis M2

50.867

96.648

0.492
  comM Acinetobacter baylyi ADP1

46.647

95.81

0.447
  pilM Legionella pneumophila strain ERS1305867

42.978

99.441

0.427

Multiple sequence alignment