Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GA636_RS04710 Genome accession   NZ_CP045135
Coordinates   970518..971237 (+) Length   239 a.a.
NCBI ID   WP_005180513.1    Uniprot ID   N9KYM2
Organism   Acinetobacter indicus strain XG01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965518..976237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA636_RS04690 - 966942..967561 (+) 620 Protein_900 IS607 family transposase -
  GA636_RS04695 - 967558..968715 (+) 1158 WP_016659040.1 RNA-guided endonuclease InsQ/TnpB family protein -
  GA636_RS04700 pilM 968864..969886 (+) 1023 WP_160232596.1 pilus assembly protein PilM Machinery gene
  GA636_RS04705 pilN 969886..970521 (+) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  GA636_RS04710 pilO 970518..971237 (+) 720 WP_005180513.1 type 4a pilus biogenesis protein PilO Machinery gene
  GA636_RS04715 pilP 971237..971764 (+) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  GA636_RS04720 pilQ 971825..973963 (+) 2139 WP_171502374.1 type IV pilus secretin PilQ family protein Machinery gene
  GA636_RS04725 aroK 974000..974542 (+) 543 WP_075167858.1 shikimate kinase AroK -
  GA636_RS04730 aroB 974565..975647 (+) 1083 WP_160232278.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 27087.71 Da        Isoelectric Point: 5.1311

>NTDB_id=392217 GA636_RS04710 WP_005180513.1 970518..971237(+) (pilO) [Acinetobacter indicus strain XG01]
MSLDQYEEMNQDPVAAPKKKMTLDKFLQQWNTLDPNNYGSWPFSVKLTIWIFIIILIAFLGYFLAIKPKLDAISNARAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLQQLPKETEIPGLVEDVNVTGVNSGLRFKNIRLENEIKQEFFIEQP
ISIEATGDYHAFGSFVSGTAALPRIVTMHDFTITAGENKEKKTDIPQITYVAKAKTYRYVGNTDAQAGNSTTPATGAAQ

Nucleotide


Download         Length: 720 bp        

>NTDB_id=392217 GA636_RS04710 WP_005180513.1 970518..971237(+) (pilO) [Acinetobacter indicus strain XG01]
ATGAGTCTCGATCAATATGAAGAAATGAATCAGGATCCTGTTGCTGCGCCAAAGAAAAAAATGACGCTGGATAAATTCCT
GCAGCAATGGAATACCTTAGATCCGAATAACTATGGCAGCTGGCCGTTTTCCGTCAAGCTGACCATCTGGATCTTTATCA
TTATTTTAATTGCCTTCCTGGGCTATTTCCTGGCCATCAAGCCGAAGCTGGATGCAATTTCCAATGCGCGTGCACAAGAG
CAGAACCTGCTGAATGAGTTTCGTGAAAAAGATTCCAAACTGCGTAACTTGCAGCAATATCAGGCGCAGTTACAGGAAAT
GGAAGCCAACTTTAACCAGCAGCTACAACAGTTGCCAAAAGAAACCGAAATTCCAGGTCTGGTTGAAGATGTGAACGTTA
CGGGTGTGAACTCAGGCCTAAGATTTAAAAATATCCGTCTGGAAAATGAAATCAAGCAAGAGTTCTTTATCGAGCAGCCA
ATTTCGATTGAAGCCACCGGTGATTATCACGCTTTTGGTTCTTTTGTGAGTGGTACAGCTGCTTTACCGCGAATTGTGAC
CATGCATGATTTCACTATTACTGCTGGTGAAAACAAGGAAAAGAAAACCGATATTCCACAAATTACCTATGTCGCGAAAG
CCAAGACCTATCGCTATGTCGGGAATACCGATGCACAGGCCGGTAATAGTACGACTCCAGCCACAGGAGCAGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9KYM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.748

99.582

0.695
  comO Acinetobacter baylyi ADP1

67.213

100

0.686

Multiple sequence alignment